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Frontiers in Plant Science logoLink to Frontiers in Plant Science
. 2022 May 18;13:836665. doi: 10.3389/fpls.2022.836665

Comparative Metabolic Analysis Reveals a Metabolic Switch in Mature, Hydrated, and Germinated Pollen in Arabidopsis thaliana

Jiang Wang 1,2,, Shrikaar Kambhampati 3,, Doug K Allen 3,4, Li-Qing Chen 1,2,*
PMCID: PMC9158543  PMID: 35665175

Abstract

Pollen germination is an essential process for pollen tube growth, pollination, and therefore seed production in flowering plants, and it requires energy either from remobilization of stored carbon sources, such as lipids and starches, or from secreted exudates from the stigma. Transcriptome analysis from in vitro pollen germination previously showed that 14 GO terms, including metabolism and energy, were overrepresented in Arabidopsis. However, little is understood about global changes in carbohydrate and energy-related metabolites during the transition from mature pollen grain to hydrated pollen, a prerequisite to pollen germination, in most plants, including Arabidopsis. In this study, we investigated differential metabolic pathway enrichment among mature, hydrated, and germinated pollen using an untargeted metabolomic approach. Integration of publicly available transcriptome data with metabolomic data generated as a part of this study revealed starch and sucrose metabolism increased significantly during pollen hydration and germination. We analyzed in detail alterations in central metabolism, focusing on soluble carbohydrates, non-esterified fatty acids, glycerophospholipids, and glycerolipids. We found that several metabolites, including palmitic acid, oleic acid, linolenic acid, quercetin, luteolin/kaempferol, and γ-aminobutyric acid (GABA), were elevated in hydrated pollen, suggesting a potential role in activating pollen tube emergence. The metabolite levels of mature, hydrated, and germinated pollen, presented in this work provide insights on the molecular basis of pollen germination.

Keywords: untargeted metabolomics, in vitro pollen germination, hydrated pollen, germinated pollen, metabolites, mature pollen, starch and sucrose metabolism

Introduction

Successful pollination in flowering plants is essential to fertilization and seed formation and is a key determinant of seed yield (Johnson et al., 2019). Mature pollen in most plant species is metabolically dormant before anthesis with approximately 15–35% water content (Shi and Yang, 2010). Pollen viability correlates with the degree of dehydration and the composition of carbohydrate and lipid reserves (Shi and Yang, 2010). Upon interacting with stigma, compatible pollen grains will undergo a rapid rehydration process that is a prerequisite to germination (Moon and Jung, 2020). It takes less than 5 min for pollen to germinate in many monocot species (Heslop-Harrison, 1979; Chen et al., 2008). By contrast, dicot pollen can take an hour to hydrate before germination (Rotsch et al., 2017). Defects in pollen hydration may result in precocious germination in anthers (Johnson and McCormick, 2001) and cause sterility (Fiebig et al., 2000). After hydration, metabolism initiates pollen tube growth from the aperture, which is controlled by multiple cellular and molecular processes (Kim et al., 2019). The rapid growth of pollen tubes is an energy-demanding process that requires mobilization of storage reserves in pollen grains (Goetz et al., 2017) and support from stigma exudates (Goldman et al., 1994; Wolters-Arts et al., 1998) coordinated by a complicated change of metabolic dynamics, protein synthesis, cell signaling, cell-wall remodification, and new cell component biosynthesis (Shi and Yang, 2010; Johnson et al., 2019; Kim et al., 2019; Hafidh and Honys, 2021).

Studies performed with rice and Arabidopsis [see review in Moon and Jung (2020) and references therein] analyzed the complexity of biochemical mechanisms initiated during pollen germination and pollen tube growth. In contrast to the wealth of available transcriptome and proteome data, global metabolomic dynamics have not been well-characterized during pollen germination. In particular, knowledge of metabolomic changes during pollen hydration is nearly absent from the literature and metabolite reports on pollen are limited to a small number of specific compounds. Secondary metabolites have been examined in tomato plants during pollen development under heat stress (Paupiere et al., 2017) and sucrose and starch catabolism has been measured in different fractions in the lily (Castro and Clément, 2007). Comprehensive metabolomic analysis during pollen germination has been performed in lilies and tobacco, two bicellular pollen species (Obermeyer et al., 2013; Rotsch et al., 2017), and the Chinese fir, a gymnosperm (Fragallah et al., 2018), but no such studies have been reported with the model plant Arabidopsis, a tricellular pollen species, despite the extensive transcriptomic resources available for comparative analysis.

In this study, we examined changes in Arabidopsis pollen by surveying metabolite profiles among mature, hydrated, and germinated pollen with an untargeted metabolomic approach. Monosaccharide, polysaccharide, sugar phosphate, lipid, and fatty acid levels changed during the process. In addition, integrated analysis of the metabolomic and publicly available transcriptomic data revealed that sucrose and starch metabolism were significantly elevated in pollen hydration and germination. Several genes that encode transporters and enzymes for phosphorylated sugars as well as lipid synthesis enzymes were differentially expressed in accordance with the changes of their metabolic intermediates, suggesting some concordance with transcript data. The potential roles of several metabolites that were over-represented in hydrated pollen required for the metabolic activation of pollen tube emergence were discussed with reference to transcriptomic data.

Materials and Methods

Plant Growth and Pollen Collection

The Arabidopsis Col-0 plants were grown under controlled temperature (22°C) with a 16-h light (100–150 μmol m–2 s–1)/8-h dark photoperiod. The pollen harvesting method, in vitro germination medium, and sampling stage for hydrated pollen (45 min after germination) and germinated pollen (4 h after germination) for metabolomic analyses were identical to methods published based on transcriptome analysis of Arabidopsis pollen germination (Wang et al., 2008), to enable comparisons. For each repeat, mature pollen grains from the fully opened flowers were collected from more than 1,000 plants using a vacuum cleaner method (Johnson-Brousseau and McCormick, 2004) at around 5 h into the light period.

Culture of Arabidopsis Pollen in vitro

For mature pollen samples, collected pollen grains were resuspended in 2 ml of ice-cold Pollen Isolation Buffer [PIB, composed of 100 mM NaPO4, pH 7.5, 1 mM EDTA, and 0.1% (v/v) Triton X-100] right after collection followed by centrifuging at 15,000 g for 1 min (4°C). For hydrated pollen and germinated pollen samples, in brief, pollen pellets were washed with 1 ml of liquid Pollen Germination Medium [PGM, composed of 15% (w/v) sucrose, 1.5 mM boric acid, 0.8 mM MgSO4, 1 mM KCl, 5 mM MES, 0.05% (w/v) lactalbumin hydrolysate, 10 μM myo-inositol, 5 mM CaCl2] before they were resuspended in 30 μl of liquid PGM and subsequently cultured in Petri dishes (35 mm in diameter). A 70 μm mesh was used to cover the pollen droplet to create a thin layer for optimal germination for each Petri dish. The Petri dishes were covered and placed in the dark for 45 min or 4 h and collected as hydrated pollen or germinated pollen, respectively. All pollen samples were washed by 1 ml ice-cold ddH2O three times before being stored in a -80°C freezer.

Starch Staining of in vitro Germinated Pollen

Mature, hydrated, and germinated pollen were prepared as aforementioned. Pollen samples were stained using 100 μl iodine solution [composed of 4% (w/v) potassium iodide and 1.27% (w/v) iodine] kept in the dark for 10 min and washed twice using 1 ml ddH2O before imaged with a compound microscope (Nikon, NY, United States).

Total Metabolite Extraction

Total metabolites from pollen were extracted using a phase separation method previously described (Kambhampati et al., 2021) with slight modifications. Briefly, 10–30 mg (fresh weight) pollen tissue, collected in Eppendorf tubes, was extracted using 700 μL of chilled 7:3 (v/v) methanol: chloroform spiked with 50 μM each of 1.4-piperazinediethanesulfonic acid (PIPES), ribitol, and norvaline as internal standards. After two metal beads were also added to the samples, they were homogenized using a Tissue-Lyser for 5 min at 30 Hz. The samples were incubated on a rotary shaker at 4°C for 2 h after which 300 μL ddH2O was added. The samples were then centrifuged at 14,000 rpm for 10 min to achieve phase separation and the upper aqueous phase, as well as the lower organic phase, were collected separately. The aqueous phases containing polar and non-polar metabolites were split into two equal parts and dried using a speed vacuum centrifuge (Labconco, Kansas City, MO, United States). The two dried parts were re-suspended in 50 μL 80% (v/v) methanol, and 30% (v/v) methanol for metabolomics analyses using hydrophilic interaction chromatography (HILIC) and reverse phase chromatography, respectively. The organic phase was also dried using a speed vacuum centrifuge and re-suspended in 50 μL of 49:49:2 (v/v/v) mixture of acetonitrile:methanol:chloroform. All samples were filtered using a 0.8 μM PES membrane centrifuge filter (Sartorius, Goettingen, Germany) and transferred to a glass vial for injection into an LC-MS/MS system.

Liquid Chromatography-Tandem Mass Spectrometry

Three different chromatographic methods, including a HILIC, a reverse phased C18 and a reverse phased C8 columns, were used to attain a wide coverage of compound groups. The aqueous fraction of the extraction, which is expected to contain core and specialized metabolic intermediates, was used for HILIC and C18 chromatography, while the organic fraction was used for C8 based chromatography for the separation of lipids. An Eksigent Ekspert microLC 200-chromatography system (Eksigent Technologies, Redwood City, CA, United States) and a CTC Analytics Leap HTS PAL liquid handler hooked to a benchtop Q-Exactive Orbitrap MS (Thermo Scientific, Waltham, MA, United States) were used for all untargeted LC-MS analysis. HILIC separation was achieved using a custom made zic-pHILIC (100 × 0.5 × 3 μm) column obtained from Higgins Analytical Inc. (Mountain View, CA, United States) with the mobile phases, 10 mM ammonium bicarbonate in ddH2O (solvent A) and 10 mM ammonium bicarbonate in 95:5 (v/v) Acetonitrile: ddH2O (solvent B) and a flow rate of 15 μL/min. The following gradient was used for HILIC; 0-2 min at 100% B, 3 min at 85% B, 16 min at 50% B, 17 min at 30% B, 18 min at 30% B, 20 min back to 100% B and equilibration up to 30 min. Reverse-phase chromatography was performed using a Targa C18 (100 × 0.3 × 5 μm) column with the mobile phases, 0.1% formic acid in ddH2O (Solvent A) and 0.1% formic acid in Acetonitrile (Solvent B), and a flow rate of 15 μL/min. The gradient conditions used for the C18 method are as follows; 0-3 min at 2% B, 13 min at 100% B, 16 min at 100% B, 19 min at 2% B, and equilibration up to 30 min. For lipidomics, a custom-made C8 column (100 × 0.5 × 1.7 μm) from Higgins Analytical Inc. (Mountain view, CA, United States) was used with the mobile phases 1% 1 M ammonium acetate, 0.1% acetic acid in ddH2O (solvent A) and 1% 1 M ammonium acetate, 0.1% acetic acid in 7:3 (v/v) acetonitrile: isopropanol (solvent B), and a flow rate of 40 μL/min. The following gradient, 0–1 min at 55% B, 3 min at 75% B, 8 min at 89% B, 10 min at 99% B, 11 min at 99% B and 12 min at 55% B followed by equilibration up to 18 min, was used, which was modified from Hummel et al. (2011) to adopt to microflow.

Data for untargeted metabolomics using all three chromatographic methods were acquired for mass ranges of 70–1,000 m/z by full MS at 70,000 resolution in both positive and negative ionization modes. The automatic gain control (AGC) and maximum injection time (IT) were 5 × 105 and 100 ms, respectively. The heated electrospray ionization (HESI) source was operated with sheath gas, 15 arbitrary units; auxiliary gas, 5 arbitrary units; capillary temperature, 250°C; auxiliary gas heater temperature, 50°C; and S-lens RF level, 50. The spray voltage was 4.2 and 3.9 kV in positive and negative modes, respectively. One sample in each group was also used for the top 12 data-dependent acquisition experiments in both ionization modes to generate MS/MS datasets for compound identification. These experiments involved a full MS scan at 70,000 resolution, AGC target 5 × 105, maximum IT 100 ms and MS/MS scans at 17,500 resolution, AGC target 5 × 104, maximum IT 50, 2.0 m/z isolation window, stepped collision energy of 15, 25, and 35 eV, intensity threshold 1 × 104 and 15-s dynamic exclusion.

Metabolic Data Analysis and Integration of Available Transcriptomic Data

For data analyses, the raw data files in Thermo.RAW format obtained in the profile mode were first centroided by conversion into .mzML format using ProteoWizard (Kessner et al., 2008) with peak picking filter applied. Features were detected and a pre-processed data table was created using the program, MZmine 2.53 (Pluskal et al., 2010), data were normalized using average squared intensities available within the MZmine workflow (Katajamaa and Orešiè, 2005) to enable comparison between different samples and the peak areas were exported to a single combined data table containing 2,235 metabolomic features. Global changes in metabolome were visualized using UMAP (McInnes et al., 2018). Raw data (.mzML format) are publicly available at National Metabolomics Data Repository [NMDR; Sud et al. (2016)]. A t-test was carried out to determine the significantly changed metabolites (P < 0.05) in pollen from the hydration or germination stage when compared with the mature pollen stage. The resultant subset of features that showed significant differences were annotated using “Functional Analysis” module of MetaboAnalyst v5.0 (Pang et al., 2021). Notably, for the untargeted metabolome analysis, as the annotated metabolites for the pathway enrichment analysis were predicted using computational algorithms (Li et al., 2013), the content of a specific metabolite needs to be manually verified if it is not provided in our already verified list. Pathway enrichment analysis was performed using the “Pathway Analysis” module of MetaboAnalyst v5.0. A differentially regulated gene list was directly retrieved from supplementary materials of published transcriptomic data by Wang et al. (2008). The differentially regulated genes and the consequential metabolites during the transition from mature to hydrated pollen, and from hydrated to germinated pollen, were integrated using the “Joint Pathway Analysis” module in MetaboAnalyst v5.0 with pathway database selection of “Metabolic pathways (integrated).” “Hypergeometric Test” was used for the enrichment analysis, “Degree Centrality” was selected for the topology analysis, and “Combine p-values (pathway-level)” was set as the integration method.

Statistical Analysis

The Shapiro-Wilk test was used to test the normality of data. For the data that passed the normal distribution test, one-way ANOVA followed by multiple comparison tests (Fisher’s LSD method) was used for the difference comparisons among multiple subjects. For the data of metabolites (glucose, raffinose, stearic acid, oleic acid, IAA, and quercitin) non-normally distributed, the Kruskal-Wallis ANOVA followed by multiple comparison tests (Dunn’s test) was used. All statistical analysis was performed using OriginPro 2021b software (OriginLab Corporation, Northampton, MA, United States).

Results

Pollen Germination Stages and Statistical Analysis

Pollen germination rates were initially evaluated using a light microscope. Consistent with previous observations (Wang et al., 2008), no pollen from mature or hydrated stages was in a germinated form, while ∼52% of observed pollen germinated in the germinated pollen stage (Figure 1A). Three different chromatographic methods were used, including hydrophilic interaction chromatography (HILIC), reverse phase chromatography with a C18 column, and lipidomic profiling using a specific reverse phase C8 column. This ensured an extensive coverage of compounds and captured core central and specialized metabolites along with several lipid classes. To obtain a global overview of the metabolomic data, a UMAP (Uniform Manifold Approximation and Projection) analysis (McInnes et al., 2018), was performed to accommodate dimensionality reduction relative to PCA (Principal Component Analysis), and to visualize the data (Figure 1B). The four biological replicates for mature, hydrated, and germinated pollen were each clustered together and the clusters were easily distinguished along the UMAP1 dimension. The hydration stage was separated from the mature and germination stages as determined by the second UMAP dimension, suggesting many peak features were uniquely present and/or accumulated at distinct levels in this stage.

FIGURE 1.

FIGURE 1

Pollen germination and statistical analysis of metabolites. (A) In vitro pollen germination assay for mature pollen (M), hydrated pollen (H), and germinated pollen (G). ND: not detected. The means were calculated from multiple repeats (±SE, n = 6), with over 420 pollen grains/tubes counted in total. (B) UMAP analysis of mature pollen(M), hydrated pollen (H), and germinated pollen (G). (C) Venn diagram showing total differential accumulated metabolites in hydrated pollen vs. in mature pollen (H vs. M) and those in hydrated pollen vs. in mature pollen (G vs. M). (D) Venn diagram showing increased metabolites in hydrated pollen vs. in mature pollen (H vs. M) and those in hydrated pollen vs. in mature pollen (G vs. M). (E) Venn diagram showing decreased metabolites in hydrated pollen vs. in mature pollen (H vs. M) and those in hydrated pollen vs. in mature pollen (G vs. M). Detailed compound information used in the Venn diagrams of panels (C–E) was shown in Supplementary Table 1.

After determining the significant changes that occurred in metabolite levels (P < 0.05) between mature and hydrated pollen, and between mature and germinated pollen, we noted the significantly increased or decreased metabolites in each pair, with verified identities presented in Supplementary Table 1. Compared to mature pollen, a total of 45 and 54 metabolites were significantly altered in hydrated and germinated pollen, respectively. Of these, 27 of the same metabolites were altered in both hydrated and germinated pollen groups (Figure 1C). Where metabolite levels increased, the majority (18/23) of metabolites were found in hydrated pollen (Figure 1D), suggesting a potential role in metabolic activation before pollen tube emergence (Kim et al., 2019). Where metabolite levels decreased, 25 metabolites were found only in germinated pollen, while nine were found only in the hydrated pollen (Figure 1E).

Differential Metabolic Pathway Enrichment Analysis

The metabolite differences were subjected to pathway enrichment analysis (Figure 2) where “enrichment” refers to an overrepresentation of the number of metabolites within a pathway that are coordinately elevated or reduced in level. During the transition from mature pollen to hydrated pollen, seven metabolic pathways were overrepresented in metabolites with elevated levels. Six metabolic pathways were overrepresented in metabolites with decreased levels (Figures 2A,B). During the transition from hydrated pollen to germinated pollen, two metabolic pathways were overrepresented in metabolites with elevated levels and ten pathways were overrepresented in metabolites with reduced levels (Figures 2C,D). When mature and germinated pollen metabolites were compared, four metabolic pathways were overrepresented in metabolites with elevated levels and thirteen metabolic pathways were overrepresented in metabolites with lowered levels (Figures 2E,F). Specifically, starch and sucrose metabolism, galactose metabolism, and flavone and flavonol biosynthetic pathways had disproportionate numbers of metabolites with increased levels in both hydrated and germinated pollen. By contrast, purine and pyrimidine metabolic pathways had more metabolites that were lowered in levels in both hydrated and germinated pollen. Interestingly, the quantity of metabolites from two overrepresented pathways increased during the transition from mature pollen to hydrated pollen, and then decreased during the transition from hydrated pollen to germinated pollen. One of the two pathways was the biosynthesis of flavone and flavonol, and the other was biosynthesis of phenylalanine, tyrosine and tryptophan. Both pathways had over-represented numbers of elevated metabolites in the hydrated pollen compared to the other two stages, suggesting their potential roles in metabolic activation for pollen tube emergence. Detailed information on pathway enrichment analysis can be found in Supplementary Table 2.

FIGURE 2.

FIGURE 2

Pathway enrichment analysis of differential metabolites from mature pollen, hydrated pollen, and germinated pollen. Pathway enrichment analysis of increased (A) and decreased (B) metabolites between mature pollen and hydrated pollen. Pathway enrichment analysis of increased (C) and decreased (D) metabolites between hydrated pollen and germinated pollen. Pathway enrichment analysis of increased (E) and decreased (F) metabolites between mature pollen and germinated pollen. For all enrichment analysis P < 0.05, the circle size corresponds to the impact of the metabolic pathway, which evaluates the ratio of matched metabolites to overall metabolites in each pathway during the pathway topology analysis.

Integrative Analysis of Transcriptomics and Metabolomics

To obtain a deeper understanding, we performed a multi-omics analysis that integrated the current metabolomic data with previously published expression levels from transcriptomics (Wang et al., 2008). As shown in Table 1, two pathways contained an overrepresented number of metabolites and genes that were either elevated or lowered in level in hydrated pollen compared to mature pollen. Three pathways contained an overrepresented number of genes and metabolites with either heightened or reduced levels in germinated pollen when compared to hydrated pollen. The list of compounds and genes from the joint analysis can be found in Supplementary Table 3. To investigate whether metabolic changes were associated with transcriptional changes, we performed a pathway enrichment analysis using the previously published transcriptome data. Unexpectedly, few of the identified pathways (e.g., linoleic acid metabolism, glycerophospholipid metabolism and purine metabolism) from our joint analysis data were significantly enriched in transcriptomics data (Supplementary Table 4). This is not surprising as disconcordance between protein and transcript levels is well-documented [Fernie and Stitt, 2012; summarized in Allen (2016)]; however, the results imply that integrative analysis can provide more insight than transcriptomics alone. Consistent with pathway enrichment analysis based only on metabolomics data, we found that starch and sucrose metabolism as well as galactose metabolism were over-represented in both hydrated and germinated pollen in the joint pathway analysis, suggesting that the compounds and enzymes related to soluble carbohydrate metabolism and cell-wall modifications were active during both pollen hydration and germination. In the published transcriptome data (Wang et al., 2008), AtSUC3 (At2g02860) and AtSUC9 (At5g06170), which encode plasma-membrane localized sucrose transporters, had increased expression in germinated pollen. AtSIP2 (At3g57520), which encodes a raffinose synthase, had elevated expression in germinated pollen. The gene expression levels were consistent with increased sucrose and raffinose that accumulated in germinated pollen (Supplementary Table 1). To investigate whether starch is altered during pollen germination, we performed starch staining using iodine solution on mature, hydrated, and germinated pollen. No clear differences were observed in the starch stains from the pollen in any of the three stages (Supplementary Figure 2), consistent with a previous report that no evidence of starch was detected in the mature Arabidopsis pollen grains after staining with iodine containing solution (Regan and Moffatt, 1990). As shown in the joint analysis (Supplementary Table 3), AtUGE1 (At1g12780), which encodes a UDP-glucose epimerase, was expressed to a higher degree in germinated pollen, and AtUGE3 (At1g63180) was also elevated in both hydrated and germinated pollen. Plant UGEs are important to the regulation of cell wall carbohydrate biosynthesis (Rösti et al., 2007), which may contribute to the heightened metabolite levels in galactose metabolism from hydrated and germinated pollen.

TABLE 1.

Joint pathway analysis of transcriptomics and metabolomics in hydrated pollen and germinated pollen.

Enriched pathway Total. compound Hits. compound Total. gene Hits. gene P Impact
Up in hydrated pollen Starch and sucrose metabolism 22 3 35 4 <0.001 0.55
Galactose metabolism 27 5 24 1 <0.001 0.36
Down in hydrated pollen Linoleic acid metabolism 63 7 78 1 0.042 0.26
Pentose and glucuronate interconversions 16 2 14 1 0.032 0.21
Up in germinated pollen Starch and sucrose metabolism 22 1 35 9 0.005 0.48
Galactose metabolism 27 2 24 3 0.035 0.54
Glutathione metabolism 26 1 24 8 0.003 0.49
Down in germinated pollen Pentose and glucuronate interconversions 16 4 14 6 <0.001 0.79
Glycerophospholipid metabolism 37 3 46 10 0.006 0.61
Purine metabolism 63 6 78 10 0.03 0.66

Total represents the total number of compounds/genes in each pathway; hits represents the number of compounds/genes that were significantly changed within each pathway.

Overview of All Differentially Accumulated Metabolites Among Mature, Hydrated, and Germinated Pollen

To give an overall comparison of metabolites among mature, hydrated, and germinated pollen, we summarized intensities in a heatmap of all manually verified metabolites (Supplementary Table 5) that passed the statistical threshold during metabolomics analysis (Figure 3). Four major clusters of metabolites were identified. The first cluster was enriched with metabolites accumulating in germinated pollen (e.g., raffinose, glucosamine). The second cluster contained metabolites enriched in hydrated pollen, including 2-aminobenzoic acid and oleic acid, and the third cluster represented metabolites elevated in mature pollen (e.g., xylose and fustin). The fourth cluster contained metabolites reduced in germinated pollen such as vitamin K2 and linoleic acid. All lipid species (including both neutral and polar lipids) were compared in a second heatmap (Supplementary Figure 1) and were mostly elevated in mature pollen but reduced in germinated pollen.

FIGURE 3.

FIGURE 3

Heatmap showing the identified metabolite differences among mature pollen, hydrated and germinated pollen. Heatmap was created using raw data in intensity converted to z-scores for each metabolite using the “pheatmap” package (Kolde, 2019) in R. The scale bar represents the distance of the raw intensity away from the group mean in units of the standard deviation for a given metabolite. Z-score is negative when the raw intensity is below the mean, positive when above.

Carbon-Related Metabolites Were Differentially Altered in Hydrated and Germinated Pollen

Sugar metabolism and fatty acid metabolism were among the enriched pathways during pollen hydration and germination. The normalized intensities of soluble sugars, fatty acids, lipids, and their adducts were retrieved from the metabolomic data (Supplementary Table 5). As shown in Figures 4A,B, glucose and fructose content were significantly elevated in hydrated pollen but unchanged in germinated pollen. By contrast, sucrose and raffinose content continued to increase during the transition from mature to hydrated to germinated pollen (Figures 4C,D). In contrast, glucose 6-phosphate, a precursor and product of sucrose metabolism, sharply decreased from mature to hydrated pollen (Figure 4E). Glucose 1-phosphate, which is also closely related to sucrose, dramatically decreased from hydrated to germinated pollen (Figure 4F). There was significantly reduced stored lipid in the form of triacylglycerol (TAG) in germinated pollen after hydration (Figure 4G). AtDGAT (At2g19450), which encodes the key diacylglycerol acyltransferase for TAG biosynthesis (Routaboul et al., 1999), was reduced in expression during pollen germination (Supplementary Table 3). Other lipid species (e.g., diacylglycerol) were also reduced during pollen germination (Supplementary Figure 1). Non-esterified fatty acids including linoleic acid and linolenic acid, the two most abundant unsaturated fatty acids found in Arabidopsis flowers (Li-Beisson et al., 2009), decreased in germinated pollen after pollen hydration (Figure 4H). Furthermore, palmitic acid, oleic acid, and linolenic acid content peaked in hydrated pollen before decreasing during pollen germination (Figure 4H), suggesting they may be involved in metabolic activation during pollen hydration. The lack of the three major fatty acids (palmitic acid, oleic acid and linolenic acid) in the pollen coat is known to result in rapid dehydration of rice pollen grains (Xue et al., 2018). Taken together, the data suggests that lipids stored in mature pollen catabolized to support the energy-demanding pollen germination process. Saccharides, including sucrose and raffinose, accumulated as pollen germination progressed, likely due to the carbon supply from the external sucrose-rich medium provided during in vitro germination.

FIGURE 4.

FIGURE 4

Central carbon intermediates changes among mature pollen (M), hydrated (H) and germinated (G) pollen. (A) Glucose, (B) Fructose, (C) Sucrose, (D) Raffinose, (E) Glucose-6-phosphate, (F) Glucose-1-phosphate, (G) total triacylglycerides, (H) Fatty acids content in mature, hydrated, and germinated pollen. The means (± SE, n = 4) were plotted. The statistically significant differences among mature, hydrated, and germinated pollen for a particular compound were represented by different letters (P < 0.05).

Amino Acids, Hormone, and Flavonoids Changes in Hydrated and Germinated Pollen

In contrast to central carbon intermediates, the levels of detected amino acids (Val, Tyr, Trp, Pro, Phe, Leu/Ile, Gln, Glu; Supplementary Table 5) did not change significantly within the three pollen stages (Figure 5A). γ-aminobutyric acid (GABA) showed a special pattern (Figure 5B), increasing in hydrated pollen followed by a sharp decrease to base level in germinated pollen. The levels of all identified compounds involved in nucleotide metabolism, including adenosine monophosphate (AMP), guanosine monophosphate (GMP), cytidine monophosphate (CMP), guanosine, adenosine, uridine, cytosine, cytidine, guanine, adenine, uracil (Figure 3), significantly decreased from mature pollen to hydrated pollen and remained low in germinated pollen (Figure 5C). Decreases in uracil and uridine in transition from mature pollen to germinated pollen suggest that the mature pollen grain is primed for rapid translation and protein synthesis upon germination. Indoleacetic acid (IAA) content also declined significantly from mature to hydrated pollen and remained at a low level in germinated pollen (Figure 5D). GABA, quercetin and the flavonoids luteolin/kaempferol sharply increased in hydrated pollen before returning to a low level in germinated pollen (Figures 5E,F) and may be related to metabolic activation for pollen tube emergence.

FIGURE 5.

FIGURE 5

Amino acids, hormone, and flavonoids changes among mature pollen (M), hydrated (H) and germinated (G) pollen. (A) Amino acids, (B) GABA, (C) Nucleotides, (D) IAA, (E) Quercetin, (F) Luteolin/Kaempferol content in mature, hydrated, and germinated pollen. The means (±SE, n = 4) were plotted. The statistically significant differences among mature, hydrated, and germinated pollen for a particular compound were represented by different letters (P < 0.05).

Discussion

Before anthesis, pollen undergoes a maturation process which includes dehydration and accumulation of storage reserves. The degree of dehydration is highly correlated with carbohydrates and lipid levels in mature pollen (Shi and Yang, 2010). However, the form of carbohydrates and dehydration status in mature pollen grains varies among plant species (Pacini, 1996; Pacini et al., 2006). Grass pollen grains generally contain a low level of water, a high level of starch, and are short-lived (Shi and Yang, 2010; Kim et al., 2019) compared to dicot pollen grains (Arabidopsis), which accumulate a high level of lipids instead of starch (Kuang and Musgrave, 1996; Ischebeck, 2016). It has been well-documented that Arabidopsis mature pollen is virtually starch-free (Regan and Moffatt, 1990; Kuang and Musgrave, 1996; Tang et al., 2009; Streb and Zeeman, 2012), which is consistent with the lack of clear starch stains in mature, hydrated, and germinated pollen (Supplementary Figure 2). In addition to changes in composition, pollen metabolism responds rapidly upon interacting with stigma cells including translation of mRNA and activation of stored enzymes to engage pollen hydration (Shi and Yang, 2010). Compared to those in mature pollen grain, metabolites from a number of central carbon biosynthetic pathways, e.g., purine and pyrimidine metabolism and fatty acids biosynthesis, were significantly reduced in hydrated pollen or germinated pollen, possibly due to increased turnover (Figures 2B,D,F), and consistent with the use of fatty acids (Figure 4H) and nucleotides (Figure 5C)/purines (Figure 3) that are building blocks for growth and were detected at higher levels in mature pollen relative to hydrated or germinated pollen.

Although Arabidopsis mature pollen is stored with lipid and carbohydrate reserves, the amount is insufficient to sustain rapid pollen tube growth given its small pollen grain size/volume (De Storme et al., 2013) and additional supplies of sugars are needed during pollen tube growth (Reinders, 2016). This concept was supported with our observations from metabolomic data and the integration of metabolome and transcriptome. We found elevated levels of starch and sucrose metabolism during the transition from mature to hydrated pollen (Figure 2A and Table 1) and from hydrated to germinated pollen (Figure 2C and Table 1), suggesting soluble carbohydrate metabolism is most active in germinated followed by hydrated and mature pollen, respectively. The elevated soluble carbohydrate metabolism can contribute to elevated sucrose and raffinose in germinated pollen (Figures 4C,D). The elevated demand for sucrose during pollen germination can be accommodated by expression of sucrose transporters like AtSUC1 (At1g71880), which when absent result in a compromised pollen germination phenotype in mutants without affecting fatty acid content (Sivitz et al., 2008). By contrast, both monosaccharides (glucose and fructose; Figures 4A,B) and di- and trisaccharides (sucrose and raffinose; Figures 4C,D) contributed to elevated carbohydrate levels in hydrated pollen. Interestingly, there were significantly higher levels of glucose and fructose in hydrated pollen and maintained at a similar level in germinated pollen after hydration. Based on targeted metabolite quantifications, elevated glucose and fructose were also found in hydrated Arabidopsis pollen compared to mature pollen (Wang et al., 2022).

Monosaccharides, including glucose and fructose, do not support in vitro pollen germination of Arabidopsis, while di- and trisaccharides, including sucrose and raffinose, can support in vitro pollen germination of Arabidopsis (Hirsche et al., 2017). Because only sucrose was supplied in the in vitro germination medium, the elevated glucose and fructose level in hydrated pollen were likely due to sucrose hydrolysis. Plasma-membrane localized sucrose transporters, such as AtSUC1 (Stadler et al., 1999; Sivitz et al., 2008), and alkaline/neutral invertases, such as A/N-InvH (At3g05820) that hydrolyze sucrose into fructose and glucose, are highly expressed in pollen (Wang et al., 2008) and atsuc1 and invh mutants exhibit reduced pollen germination and fewer seeds per silique, respectively (Sivitz et al., 2008; Battaglia et al., 2017). When cell-wall invertase activity has been reduced via RNA-interference or an invertase inhibitor, pollen germination rates and seed sets are reduced in both A. thaliana and N. tabacum (Hirsche et al., 2009). Soluble carbohydrates, especially glucose, are potent signaling molecules involved in many aspects of plant growth (Rolland et al., 2006); thus, the accumulated glucose and fructose in hydrated pollen may initiate signaling events that result in metabolic activation before pollen tube emergence. Increased glucose levels were not observed in germinated pollen, suggesting pollen metabolism limits glucose levels to avoid pollen tube growth inhibition known to occur at high glucose concentrations (Rottmann et al., 2018).

In our results, almost all lipid species accumulated to high levels in mature pollen and were subsequently reduced in germinated pollen (Supplementary Figure 1), consistent with the lipid accumulation pattern that was observed in tobacco pollen (Dorne et al., 1988). Neutral lipids including TAG in tobacco pollen were reduced during 6 h of pollen germination, similar to lipid bodies in olive pollen after 7 h of pollen germination (Rodriguez-Garcia et al., 2003). Another study detailed changes in neutral and polar lipid fractions over olive pollen germination (Hernández et al., 2020). Interestingly, they found the fatty acid composition from total lipids was significantly altered, although the TAG content remained unchanged during the first 6 h of germination (Hernández et al., 2020). These results indicate lipid dynamic changes are stage- and species-dependent. As widely reported, an external supply of sucrose is required for in vitro Arabidopsis pollen germination, though the presence or absence of sugars had little impact on in vitro olive pollen germination or pollen tube growth rates (Zienkiewicz et al., 2013), possibly suggesting lipid reserves are the primary carbon source for olive pollen germination without significant requirement for sugars. On the other hand, a different pattern in sugar accumulation (relative to our study) was reported during tobacco pollen germination (Rotsch et al., 2017). To be specific, sucrose decreased sharply after pollen rehydration although remaining low during pollen germination, while fructose progressively increased as pollen germination proceeded. Tobacco pollen can also germinate without any sugars (Rotsch et al., 2017), suggesting that lipid and sucrose reserves in tobacco pollen are sufficient for subsequent germination. Thus, changes in metabolite levels, such as sugars and lipids, over the pollen germination stages are highly species-dependent and will require further investigations to understand the differences in mechanisms underlying in vitro pollen germination.

The GABA content peaked in hydrated pollen (Figure 5B). In plants, GABA levels are regulated by stress, signaling, energy production and play a major role in balancing carbon/nitrogen metabolism by linking amino acid metabolism and the TCA cycle through the GABA shunt (Fait et al., 2008). Exogenous GABA stimulates Arabidopsis pollen tube growth at low concentrations but inhibits pollen tube growth at high concentrations during in vitro pollen germination (Palanivelu et al., 2003), likely through GABA-gated aluminum-activated malate transporter (ALMT) (Ramesh et al., 2015). The mutant of the POP2 gene, which encodes a transaminase that degrades GABA, accumulates a high level of GABA and the pollen tube growth of pop2 is arrested (Palanivelu et al., 2003), suggesting the GABA level plays a critical role in pollen tube growth/signaling and similar GABA patterns in lily and tobacco pollen germination (Obermeyer et al., 2013; Rotsch et al., 2017) may suggest conserved roles in development across some species.

Auxin is one of the most important hormones to promote cell division and elongation in plants (Zhao, 2010). Auxin plays a critical role in Arabidopsis pollen maturation (Cecchetti et al., 2008; Salinas-Grenet et al., 2018), but external auxin treatment reduced in vitro pollen germination rate of Arabidopsis (Ding et al., 2012). Our observations that IAA is accumulated at high levels in mature pollen, while barely detectable in hydrated pollen and germinated pollen (Figure 5D) is consistent with the prior descriptions. However, external IAA can stimulate in vitro pollen tube growth of Nicotiana tabacum (Chen and Zhao, 2008) and Torenia fournieri (Wu et al., 2008), suggesting the role of auxin during pollen tube growth is species-dependent.

The role of flavonoids during pollen germination also varies among different plant species. A male sterile phenotype of flavonoid-deficient mutant was observed in maize (Coe et al., 1981) and petunia (Ylstra et al., 1994), but Arabidopsis plants that are deficient in flavonoid biosynthesis appear to be fully fertile (Burbulis et al., 1996). As common flavonoids in plants, quercetin and luteolin/kaempferol accumulate to high levels in hydrated pollen (Figures 5E,F), flavone and flavonol biosynthesis was engaged and resulted in elevated metabolite levels during pollen hydration (Figure 2A). Our results support flavonoid involvement in the onset of pollen germination, but its role on pollen fertility may be limited in Arabidopsis.

As already mentioned, the germinated pollen samples object of this study consisted of both non-germinated and germinated pollen, with an overall germination rate of ∼52%, comparable to previously published rates for Arabidopsis [39% in Sivitz et al. (2008); 58% in Qin et al. (2009); 48.9% in Hirsche et al. (2017)]. Pollen germination is a consequence of both the rate of hydration and the speed at which the pollen tube tip is able to accomplish tube emergence (Firon et al., 2012). The fact that a substantial number of pollen grains failed to germinate in vitro indicates issues connected to the hydration process in non-germinable pollen grains. Although it would be possible to largely separate pollen tubes from non-germinated pollen grains using a 50-μm nylon mesh (Wang et al., 2008), we faced the impossibility of sufficiently remove these non-germinable pollen grains from the hydrated pollen samples on large scale. Thus, what we called germinated pollen samples were mixed samples (germinated and non-germinated) in order to compare them to the mixed samples of hydrated pollen (germinable and non-germinable) and to already published results with similar germination rates. Previously reported metabolomic (Obermeyer et al., 2013; Fragallah et al., 2018) and transcriptomic (Qin et al., 2009) studies on germinated pollen followed a really similar approach, thus, increasing our confidence on the results obtained.

To conclude, a comprehensive metabolome analysis during Arabidopsis pollen germination in combination with published transcriptome revealed a complicated metabolic pathway network in support of pollen hydration and germination. The detailed analyses of carbohydrates and fatty acids indicated their roles in carbon metabolism that varied in mature, hydrated, and germinated pollen stages and a unique set of metabolites were identified to accumulate in the hydrated pollen stage, but were barely accumulated in other two stages.

Data Availability Statement

Raw and pre-processed metabolomic data is available at the NIH Common Fund’s National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org where it has been assigned Study ID ST002060. The data can be accessed directly via its Project doi: 10.21228/M8570V.

Author Contributions

JW and L-QC conceived and designed the experiments. JW and SK conducted the experiments and performed the metabolomics analysis. DA supervised the metabolomics analysis and edited the manuscript. JW, SK, and L-QC wrote the manuscript. All authors contributed to the article and approved the submitted version.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Acknowledgments

We would like to acknowledge the Proteomics and Mass Spectrometry Core Facility at the Danforth Center where data for metabolomics were collected and Brad Evans, Russell Williams, and Michael Wei for instrument support and maintenance.

Funding

This research was supported by startup funds from the University of Illinois at Urbana–Champaign (to JW, and L-QC). DA was supported by the USDA-ARS and SK by the National Institute of Health (U01 CA235508). Establishment of preliminary conditions for subsequent method development using QTRAP6500 was supported by NSF-MRI funds (DBI-1427621).

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2022.836665/full#supplementary-material

Supplementary Figure 1

Heatmap showing the identified lipid species differences among mature pollen, hydrated and germinated pollen. Heatmap was carried out on raw intensity data converted to z-scores for each metabolite using the “pheatmap” package in R. The scale bar represents the distance between raw intensity and the population mean in units of the standard deviation for a given metabolite. Z-score is negative when the raw intensity is below the mean, positive when above. DG, diacylglycerol; TG, triacylglycerol; PA, phosphatidic acid; PC, phosphatidylcholine; PI, phosphatidylinositol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine.

Supplementary Figure 2

Starch staining of mature, hydrated, and germinated pollen. Mature, hydrated, and germinated pollen collected from 50 flowers were stained using iodine solution. No clear starch stains (dark purple) were observed from mature, hydrated, and germinated pollen.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Figure 1

Heatmap showing the identified lipid species differences among mature pollen, hydrated and germinated pollen. Heatmap was carried out on raw intensity data converted to z-scores for each metabolite using the “pheatmap” package in R. The scale bar represents the distance between raw intensity and the population mean in units of the standard deviation for a given metabolite. Z-score is negative when the raw intensity is below the mean, positive when above. DG, diacylglycerol; TG, triacylglycerol; PA, phosphatidic acid; PC, phosphatidylcholine; PI, phosphatidylinositol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine.

Supplementary Figure 2

Starch staining of mature, hydrated, and germinated pollen. Mature, hydrated, and germinated pollen collected from 50 flowers were stained using iodine solution. No clear starch stains (dark purple) were observed from mature, hydrated, and germinated pollen.

Data Availability Statement

Raw and pre-processed metabolomic data is available at the NIH Common Fund’s National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org where it has been assigned Study ID ST002060. The data can be accessed directly via its Project doi: 10.21228/M8570V.


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