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. 2022 Mar 16;13(4):8076–8086. doi: 10.1080/21655979.2022.2052672

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Upregulation of E2F1 was observed in breast cancer. (a) RPL5 expression data were acquired from StarBase database. (b) E2F1 mRNA expression levels were determined in paired tumor tissues and para-carcinoma tissues (n = 30) by qPCR. (c) E2F1 expression was detected in normal and tumor tissues using IHC assay. (d) The correlation between E2F1 and RPL5. (e) E2F1 mRNA expression levels were determined using qPCR in MCF-10A, MCF-7, BT-549, and MDA-MB-231 cells. (f) The mRNA expression levels of E2F1 after overexpressing RPL5 were estimated by qPCR. (g) The protein levels of E2F1 were measured by western blotting. **P < 0.01. ***P < 0.001.