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. 2022 Mar 25;13(4):8605–8616. doi: 10.1080/21655979.2022.2053814

Figure 4.

Figure 4.

Effects of TTTY15/let-7a-5p axis on GC cell proliferation, apoptosis, migration and invasion. (a) qRT-PCR was used to detect the transfection efficiency of TTTY15 overexpression plasmids + miR mimics and si-TTTY15-1 + miR inhibitors. (b and c) CCK-8 method and BrdU assay were used to detect the effects of TTTY15 and let-7a-5p on the viability and proliferation of NCI-N87 and SUN-1cells. (d) Flow cytometry assay was used to detect the effects of TTTY15 and let-7a-5p on NCI-N87 and SUN-1 cell apoptosis. (e and f) Transwell assays were used to detect the effects of TTTY15 and let-7a-5p on the migration and invasion of NCI-N87 and SUN-1 cells. (g) Western blot was used to detect the effects of TTTY15 and let-7a-5p on the EMT process of NCI-N87 and SUN-1 cells. All of the experiments were performed in triplicate. ** P < 0.01 and *** P < 0.001.