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. 2022 Mar 21;13(4):8101–8114. doi: 10.1080/21655979.2022.2051783

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — NPY2R-activated MAPK signaling pathway is associated with microglia apoptosis. (a) The mRNA and protein levels of NPY2R in MN9D, C6, and BV-2 cells determined by RT-qPCR (left) and Western blot assay (right). (b) The knockdown effect of three shRNAs targeting NPY2R in BV-2 cells determined by RT-qPCR (left) and Western blot assay (right). (c) The expression effect of three NPY2R overexpression plasmids in BV-2 cells determined by RT-qPCR (left) and Western blot assay (right). (d) The viability of BV-2 cells in response to NPY2R overexpression or knockdown as examined by CCK-8 assay. (e) The viability of BV-2 cells in response to NPY2R overexpression or knockdown as examined by EdU assay. (f) The effect of NPY2R overexpression on the expression of MAPK signaling pathway related factors (ERK, p38, and JNK) in response to NPY2R overexpression or knockdown as determined by Western blot assay. (g) The apoptosis of BV-2 cells in response to NPY2R overexpression or knockdown as as examined by flow cytometry. Cell experiments were repeated three times independently.