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Astragaloside-IV (AS-IV) alleviates the damage caused by high glucose by reducing Sirt1/Nrf2 signaling activity in retinal pigment endothelial (RPE) cells. (a, d) Detection of Nrf2 by Western blot analysis. (b, c) Detection of Sirt1 by Western blot analysis. (e) Levels of glutathione peroxidase 4 (GPX4), glutamate cysteine ligase catalytic subunit (GCLC), and glutamate cysteine ligase modifier subunit (GCLM) proteins detected by Western blot analysis. ** indicates p values < 0.01.
