Correction for Aramesh et al., Nanoconfinement of microvilli alters gene expression and boosts T cell activation

Applied Biological Sciences, Biophysics and Computational Biology Correction for “Nanoconfinement of microvilli alters gene expression and boosts T cell activation,” by Morteza Aramesh, Diana Stoycheva, Ioana Sandu, Stephan J. Ihle, Tamara Zünd, Jau-Ye Shiu, Csaba Forró, Mohammad Asghari, Margherita Bernero, Sebastian Lickert, Annette Oxenius, Viola Vogel, and Enrico Klotzsch, which published October 1, 2021; 10.1073/pnas.2107535118 (Proc. Natl. Acad. Sci. U.S.A. 118, e2107535118).

The authors note that Mateusz Kotowski and Simon J. Davis should be added to the author list and credited with contributing new reagents/analytic tools. The corrected author line, affiliation line, and author contributions appear below.

Morteza Aramesh^a,1, Diana Stoycheva^a, Ioana Sandu^b, Stephan J. Ihle^c, Tamara Zünd^a, Jau-Ye Shiu^a,d, Csaba Forró^e,f, Mohammad Asghari^g, Margherita Bernero^a, Sebastian Lickert^a, Mateusz Kotowski^h, Simon J. Davis^h, Annette Oxenius^b, Viola Vogel^a and Enrico Klotzsch^a,i,1

Author affiliations: ^aLaboratory of Applied Mechanobiology, Department for Health Sciences and Technology, ETH Zürich, Zürich, 8093, Switzerland; ^bInstitute of Microbiology, Department of Biology, ETH Zürich, Zürich, 8093, Switzerland; ^cLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, Zürich, 8092, Switzerland; ^dGraduate Institute of Biomedical Sciences, China Medical University, Taichung, 40402, Taiwan; ^eDepartment of Chemistry, Stanford University, Stanford, CA 94305; ^fTissue Electronics, Fondazione Istituto Italiano di Tecnologia, 53-80125 Naples, Italy; ^gInstitute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, 8093, Switzerland; ^hRadcliffe Department of Medicine and United Kingdom Medical Research Council Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, United Kingdom; and ⁱInstitute for Biology, Experimental Biophysics/Mechanobiology, Humboldt University of Berlin, Berlin, 10117, Germany

Author contributions: M. Aramesh, D.S., A.O., V.V., and E.K. designed research; M. Aramesh, D.S., I.S., T.Z., J.-Y.S., M. Asghari, M.B., and S.L. performed research; M. Aramesh, M.K., S.J.D., A.O., V.V., and E.K. contributed new reagents/analytic tools; M. Aramesh, D.S., I.S., S.J.I., T.Z., J.-Y.S., C.F., M. Asghari, A.O., V.V., and E.K. analyzed data; and M. Aramesh wrote the paper.

The authors also note the following statement should be removed from the Acknowledgments: “We thank Simon J. Davis for the kind donation of cell lines.”

The legend for Figs. S5 and S6 in the SI Appendix appeared incorrectly. The Figs. S5 and S6 legend should appear as:

“Bar diagrams show CD69 expression for Jurkat T-cells expressing full-length (full) and truncated CD45, on 200 nm-porous and nonporous surfaces (3 replicates, left). The confocal fluorescence microscopy image (top right) reveals a dotted pattern, wherein protrusion-associated truncated CD45 is visible within the 200 nm pores. Briefly (bottom right), the truncated CD45 comprised a chimera of the extracellular domain of rat CD90, and the transmembrane and cytosolic regions of human CD45. The chimeric protein was expressed in a Jurkat-derived cell line (“J8”)¹⁶ that has the NY-ESO specific TCR, 1G4, in place of the endogenous Jurkat TCR, and CD8 in place of CD4. The control line comprised J8 cells expressing full-length CD45 at levels matching those of J8 cells expressing truncated CD45. See generation of constructs and cell lines in 1-Experimental.”

In the SI Appendix, the following text was added on page 5:

“Generation of the constructs encoding full-length and truncated CD45: A full-length CD45 construct was produced by preparing DNA encoding the chicken RPTPσ signal peptide (cRPTPσSP), a hemagglutinin (HA) tag, human CD45RABC (residues 26-1306), and a SNAP-tag®, in that order. A truncated CD45 construct was generated by preparing DNA encoding cRPTPσSP, an HA tag, the rat CD90 ectodomain (residues 20-130), the transmembrane and cytosolic regions of human CD45 (residues 576-1306) and a HaloTag®. Both constructs were cloned into the pHR-SIN lentivirus expression vector. Gene synthesis and cloning were performed by Genewiz Inc. and the sequences of the final constructs verified by NGS sequencing.

Generation of cell lines expressing full-length and truncated CD45: Lentivirus was produced by cotransfecting HEK293T cells with pHR-SIN, pMD.G and p8.91. After 72 h the viral supernatant was harvested and filtered using a 0.45 µm syringe filter. Lentiviral supernatant was then added to Jurkat (J8) T-cells expressing 1G4 TCR, CD8 and LFA-1. Virus-containing medium was replaced after 24 h and, 48 h later, expression of the transgenes was assessed by flow cytometry using PE-conjugated anti-HA antibody.¹⁶”

Reference 16 in the SI Appendix appeared incorrectly. It should instead appear as: Yang H, et al., HLA-E-restricted, Gag-specific CD8+ T cells can suppress HIV-1 infection, offering vaccine opportunities. Sci Immunol. 6 (57), eabg1703 (2021).

The article and SI Appendix have been corrected online.