Immunology and Inflammation Correction for “Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcγ receptors I and II/III,” by Francisco A. Bonilla, Ross M. Fujita, Vadim I. Pivniouk, Andrew C. Chan, and Raif S. Geha, which was first published February 4, 2000; 10.1073/pnas.040543597 (Proc. Natl. Acad. Sci. U.S.A. 97, 1725–1730).
The authors note that the SLP-76 control immunoblots in the middle panels of panels A and B of Fig. 2 are duplicated. The error does not impact the conclusion that SLP-76 phosphorylation is induced by ligation of both FcγR1 and FcγRII/III (Fig. 1A). It unfortunately throws some uncertainty as whether it is also induced by individual ligation of FcγR1 and FcγRII/III. They note that the same lysate was used to probe for SLP-76 a BLNK and Syk phosphorylation following individual ligation of FcγR1 or FcγRII/III and shows comparable loading of BLNK and Syk in stimulated and control cells. This argues in support of the conclusion that individual ligation of FcγR1 or FcγRII/III induces SLP-76 phosphorylation.