Correction to: Apolipoprotein E regulates lipid metabolism and α‑synuclein pathology in human iPSC‑derived cerebral organoids

Correction to: Acta Neuropathologica (2021) 142:807–825 10.1007/s00401-021-02361-9

The original publication of this article contained an error in a Western blot image of Fig. 4b. The positive control lane of LIMP1 was mistakenly cropped when preparing the figure. The correct image with positive control is now placed in the updated Fig. 4b. This error did not affect the results nor conclusion.

Fig. 4.

ApoE2 and apoE3, but not apoE4, partially rescue α-synuclein accumulation in apoE-deficient cerebral organoids. The APOE^−/− iPSC-derived cerebral organoids at Day 90 were treated with conditioned media of immortalized astrocytes from APOE2-target replacement (TR), APOE3-TR, or APOE4-TR mice for 5 days. Conditioned media from Apoe-KO astrocytes were used as a control. a, A schematic workflow of the rescue experiments. b–i, Amounts of EEA1 (c), LAMP1 (d), GCase (e), LIMP1 (f), Plin2 (g), αSyn (h) and p-αSyn (i) in the RIPA fraction of the cerebral organoids after treatments were quantified by Western blotting. j–l, Amounts of total αSyn (k) and p-αSyn (l) in the SDS fraction of cerebral organoids after treatments were quantified by Western blotting. All data were normalized to β-actin levels. 3 cerebral organoids were pooled and analyzed as one sample. All data are expressed as mean ± SEM (n = 5 samples/each). Experiments were repeated in three independent differentiation batches. One-way ANOVA was performed to determine statistical significance. **p < 0.01, ****p < 0.0001, n.s., not significant

The corrected Fig. 4 is given below.