Figure 6.

RBD IgG⁺ children and adults have prominent TCRαβ motifs

(A) Epitope-specific populations from RBD IgG⁺ children and adults were quantified for defining features using a neighbor distance distribution. A lower distribution peak indicates a more clustered epitope-specific TCRαβ repertoire, and the average distance values for each epitope are depicted within the plot. Analyses for both single and paired chains are shown, as indicated in the plot labels. PDF, probability density function.

(B) TCR logo representations of CDR3α and β sequence motifs for RBD IgG⁺ children and adults. Each TCR chain motif depicts the V (left side) and J (right side) gene frequencies, the CDR3 amino acid sequence (middle), and the inferred rearrangement structure (bottom bars colored by source region; V-region, light gray; insertions, red; diversity (D)-region, black; and J-region dark gray). The motif scores were determined by chi-squared, with values greater than 90 considered highly significant.

(C) Probabilities of generation (P_gen; log₁₀ transformed) for all single TCRα and TCRβ chains from RBD IgG⁺ children and adults were generated with TCRdist. Box plots represent the median (middle bar), 75% quantile (upper hinge), and 25% quantile (lower hinge), with whiskers extending 1.5 times the inter-quartile range.

See also Figure S5.