Video 2. Caspase activity is required for Ca²⁺ influx induced by familial cold autoinflammatory syndrome-associated NLRP3 mutant.

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Differentiated TRE-NLRP3-L353P-THP-1 cells were loaded with 4 μM Fluo-8 for 1 hr and were pretreated with VX-765 (20 μM) for 30 min. After doxycycline (30 ng/mL) treatment, the images were recorded at 5 min intervals from 3 hr to 6 hr.