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. 2022 May 26;27(11):3425. doi: 10.3390/molecules27113425

Figure 3.

Figure 3

The effect of 0.3 mM spermidine (SPD) and 0.2 mM eugenol (EUG), alone or in combination (0.3 mM SP + 0.2 mM EUG) on LC3-II activation in SHSY5Y (A,B), HUVEC (C,D) and HBEpiC (E,F) cell lines after 4 h. LC3-II was detected by fast red chromogen staining (A,C,E) and the percentage of LC3-II positive cells reported compared to untreated control cells (B,D,F). The MTT assay for all treatments was performed and the data reported as percentage of the untreated control for (G) SHSY5Y (H), HUVEC and (I) HBEpiC cells. The number of stars *, **, *** and **** represents significant differences between treatments as determined by one-way ANOVA at the 99% confidence level (p < 0.01). The black dots indicate the positioning of the individual replicates within the bar for each sample.