Figure 7.

The effect of a 1 h pretreatment with 0.3 mM spermidine (SPD) and 0.2 mM eugenol (EUG), alone and in combination (0.1 mM SPD + 0.05 mM EUG), before the administration of lipopolysaccharide (LPS, 1ng/mL) to Caco-2 cells (A–C) and L929 cells (D–F) for 24 h (A,D). Treatments were compared with the untreated control and LPS treatment alone. Western blotting of LC3-II with respect to β-actin was performed on Caco-2 cells (A) and L929 cells (D) that were untreated or treated with LPS + SPD, LPS + EUG and LPS + SPD + EUG and the pixels quantified (B,E). Cell viability using the MTT assay on the Caco-2 (C) and L929 (F) cell lines. The number of stars *, **, *** and **** represents significant differences between treatments as determined by one-way ANOVA at the 99% confidence level (p < 0.01), whereas n.s. is not significant. The black dots indicate the positioning of the individual replicates within the bar for each sample.