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. 2021 Jul 15;71(7):1373–1385. doi: 10.1136/gutjnl-2020-323345

Figure 2.

Figure 2

TCA trafficking in HSCs. Sorted LX2NTCP+ cells were incubated with buffer containing 100 µM TCA supplemented with radiolabeled 0.25 μC [3H] TCA with or without NTCP blocker (neutralizing antibody) for 15, 30 or 45 min. Kinetics of TCA concentrations in (A) medium and (B) cell lysate made by scintillation counting. Kinetics of MFI of LX2NTCP+αSMA of 15 min time intervals following TCA insults with or without NTCP neutralizing antibody performed by (C) flow cytometry, (D) western blots of fibrosis markers (αSMA, Col I and Col III (following 30 and 45 min)) and (E) quantitated through protein:β-actin ratio. Results between groups were statistically significant (p<0.05). αSMA, alpha smooth muscle actin; MFI, mean fluorescence intensity; NTCP, sodium+/taurocholate cotransporting polypeptide; TCA, taurocholic acid.