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. Author manuscript; available in PMC: 2023 May 20.
Published in final edited form as: Vaccine. 2022 Apr 22;40(23):3244–3252. doi: 10.1016/j.vaccine.2022.03.053

Figure 1. Experimental design of the GLP toxicology study for OXY-sKLH.

Figure 1.

Groups were administered vaccine or alum adjuvant control via IM injection as designated. Group 1 received alum, Group 2 received 1.43 μg/animal/vaccination, and Groups 3 and 4 received 68 μg/animal/vaccination. Animals were vaccinated every 2 weeks for a total of 4 vaccinations. Animals in Group 4 were sacrificed one week after last immunization (Day 50) while all other groups were then challenged with oxycodone (1.47 mg/kg, s.c.) daily for 7 days. Ten male and female animals from each of the remaining groups (Groups 1 – 3) were sacrificed after the oxycodone challenge (Day 58). A subset (5 male and 5 female) of Groups 1 and 3 were kept under observation for an additional 4 week recovery period and sacrificed at the end of the recovery phase (Day 87).