Author Correction: Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

Correction to: Scientific Reports 10.1038/srep39869, published online 05 January 2017

This Article contains errors, that were not addressed in the previous correction¹.

In Figure 1E, the images for TCP at 2d, PLGA+S-0.1 at 4d, and TCP at 4d are incorrect. A corrected version of Figure 1 and its accompanying legend appear below.

Figure 1.

Effects of SDS on RSC 96 via MTT analysis in vitro. (A) Chemical structure of salidroside; (B) Preliminary drug screening analysis of RSC 96 treated on PLGA scaffold with different concentrations of salidroside after 3 days (n = 3, mean ± SEM); (C) Proliferative effects of salidroside on RSC96 on PLGA scaffold measured by MTT assay (n = 3, mean ± SEM). Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05); (D) Quantitative data of the mean number of SCs. Data of each bar are shown as the mean of three independent experiments ± SD. Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05); (E) Cell viability was measured by FDA/PI staining under microscope. (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP).

In Figure 3, the images for PLGA+S-0.1 at 4d and PLGA+S.02 at 4d in Figure 3A and TCP at 4d in Figure 3B are incorrect. A corrected version of Figure 3 and its accompanying legend appear below.

Figure 3.

Effects of SDS on RSC 96 via SEM, immunohistochemical analysis, Western Blot assay and gene expression analysis in vitro. (A) Scanning electron microscopy (SEM) of the cells on scaffolds at 4 days. (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP). Statistic analysis of scanning electron microscopy (SEM). Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05). (B) Immunohistochemical analysis for S-100 protein, RSC 96 showed positive cytoplasmic staining for S-100 at 4 days. (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP). Statistic analysis of immunohistochemical analysis. Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05). (C) Western Blot assay of S-100 protein and quantification of the proteins expression. Full-length blots are presented in supplementary information. Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05); (D) Gene expression analysis of important neurotrophic factors (GDNF, BDNF and CNTF) by qRT-PCR in six groups at 2, 4 and 6 days. The gene expression levels were analyzed by the 2^−ΔΔCT method using GAPDH as the internal control. The data represent the mean of three independent experiments (n = 3, mean ± SEM). Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05). (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP).