BMDM exclusively target L.m. by PINCA. (A-D) GFP-LC3 transgenic BMDM of the indicated genotypes were infected with wt (A-C) or Δhly L.m. (D) for the indicated periods of time. LC3+ L.m. were quantified by immunofluorescence microscopy. Data are shown as mean ± SEM of six (A), two (B), seven (C) or three (D) independent experiments. Representative micrographs from 1 h after infection are shown. Scale bar: 4 µm. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < .0001.