Figure 5.

Priming of BMDM with pro-inflammatory cytokines enhances ROS production through CYBB. WT and cybb^−/- BMDM were either left untreated or stimulated for 48 h with TNF or for 24 h or 48 h with IFNG and then infected with L.m. where indicated. (A) ROS production after infection was measured using isoluminol. The kinetics of ROS production and the AUC as a measure for the total amount of ROS produced are shown. (B) Expression levels of CYBB, CYBA, NCF2, NCF1 and NCF4 in naïve and primed BMDM were analyzed by western blot and quantified by densitometry. Data are shown as mean ± SEM of six (A) and three to thirteen (B) independent experiments. Representative immunoblots are shown. n.i., not infected; ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.