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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Nature. 2022 May 25;606(7912):146–152. doi: 10.1038/s41586-022-04783-1

Fig. 2|. CCR5 regulates the temporal window of memory linking.

Fig. 2|

a, Characterization of the temporal window for contextual memory linking (Ctx A, Context A; Ctx B, Context B; 5h n=32, 1d n=26, 2d n=14, 7d n=16 mice; *P < 0.05, one-way ANOVA).

b, CCL5 infusion in dCA1 attenuated 5h contextual memory linking (Veh n=20, CCL5 n=17 mice; *P < 0.05, ****P <0.0001, two-way repeated measures ANOVA).

c, Schematics of the Opto-CCR5 construct.

d, Schematics of viral constructs injection. Scale bar, 500 μm.

e, Optogenetic activation of neuronal CCR5 impaired 5h contextual memory linking (Control n=15, Opto-CCR5 n=14 mice; *P < 0.05, ***P < 0.001, two-way repeated measures ANOVA).

f, Left: Schematics of AAV8-shCon or AAV8-shCCR5 intrahippocampal injection. Scale bar, 500 μm. Right: Ccr5 knockdown in dCA1 neurons extended the temporal window of contextual memory linking (shRNA-Con n=13, shRNA-CCR5 n=16 mice; *P < 0.05, **P < 0.01, two-way repeated measures ANOVA).

g, Ccr5 knockout extended the temporal window of contextual memory linking (WT n=9, Ccr5+/− n=6, Ccr5−/− n=7 mice; *P < 0.05, **P < 0.01, two-way repeated measures ANOVA).

All results shown as mean ± s.e.m.