Figure 3. C. innocuum LC-CI and CI_01447 or CI_01447 cytotoxicity towards HeLa cells.
Lactate dehydrogenase release determined from HeLa cells incubated with (A) bacterial pellets or supernatant (S/N) from C. innocuum LC-CI or C. difficile RT106/DH/ST-42, or (B) recombinant protein CI_01447 and CI_01448. Percent cytotoxicity is relative to Triton X-100 positive control. Experiments were completed in at least duplicate with technical duplicates. Error bars indicate standard deviation. ns, not significant; *, p-value < 0.05.