In Table 3, the following text should be removed from the legend:
Table 3. Pairwise FST values between 9 sampling locations.
| Barrio Florida | Aucayo | Indiana/Mazan | Nauta | Tamshiaco | Iquitos A | Iquitos B | Iquitos C | Iquitos D | |
|---|---|---|---|---|---|---|---|---|---|
| Barrio Florida | 0 | ||||||||
| Aucayo | 0.0793* | 0 | |||||||
| Indiana/Mazan | 0.03882* | 0.03062* | 0 | ||||||
| Nauta | 0.03857* | 0.04908* | 0.05776* | 0 | |||||
| Tamshiaco | 0.05879* | 0.04171* | 0.05084* | 0.02182* | 0 | ||||
| Iquitos A | 0.09909* | 0.04595* | 0.05694* | 0.06006* | 0.03617* | 0 | |||
| Iquitos B | 0.03452* | 0.0221* | 0.01617* | 0.04593* | 0.01277* | 0.03446* | 0 | ||
| Iquitos C | 0.11186* | 0.05321* | 0.08134* | 0.07741* | 0.02794* | 0.05779* | 0.0453* | 0 | |
| Iquitos D | 0.03007* | 0.01994 | 0.01682 | 0.02788* | 0.00845 | 0.03074 | 0.00362 | 0.04614 | 0 |
* FST values statistically different from zero, indicating genetic differentiation (p < 0.05).
The fixation index, FST, ranges from 0 to 1 and measures the degree of genetic relatedness between two pairs of populations by comparing the variation observed in the subpopulation with the variation observed in the total population. Values approaching 0 represent panmixia, whereas values approaching 1 represent complete genetic isolation (non-interbreeding populations). Our findings show low to moderate genetic differentiation, with the greatest degree of differentiation observed for Barrio Florida (highest FST values overall) and a lower degree of differentiation for Iquitos sites (lower FST values).
Genetic variation was compared within and between populations by AMOVA. Among all sites, approximately 71.5% of the variation was attributable to differences among individuals, whereas 24.5% of the variation was explained by individuals within populations, and just 4.1% among collections within Iquitos (Table 4).
Table 4. Analysis of Molecular Variance (AMOVA) of Ae. aegypti mosquitoes using 8 microsatellite loci.
| Source of Variation | df | Sum of Squares | Variance components | Variation (%) | F-Statistic | P-value |
|---|---|---|---|---|---|---|
| Among groups | 5 | 40.895 | -0.00770 | -0.38% | FCT = 0.28534 | 0.61693 |
| Among populations within groups | 3 | 22.246 | 0.08117 | 4.05% | FSC = 0.04035 | <0.0001* |
| Among individuals within populations | 330 | 801.514 | 0.49834 | 24.87% | FIS = 0.25814 | <0.0001* |
| Within individuals | 339 | 485.500 | 1.43215 | 71.47% | FIT = 0.28534 | <0.0001* |
| Total | 667 | 1350.155 | 2.00397 |
FCT, differentiation among groups.
FSC, differentiation among populations within groups (Iquitos samples).
FIS, differentiation among individuals within populations.
FIT, differentiation within individuals.
*Statistically significant values (p < 0.05).
AMOVA results showed that 71.5% of the total variation observed was attributable to differences within individuals; only 4.1% of the total variation was attributable to differences among samples collected within Iquitos, indicating a lack of genetic structure within the city.
The above text should appear within the fourth paragraph of the Results section.
In the fourth paragraph of the Results section, the following sentence should be removed:
AMOVA results showed that 71.5% of the total variation observed was attributable to differences within individuals; only 4.1% of the total variation was attributable to differences among samples collected within Iquitos, indicating a lack of genetic structure within the city.
The above sentence should appear in the legend of Table 3. Please see Tables 3 and 4 and their correct legends here.
Reference
- 1.Guagliardo SAJ, Lee Y, Pierce AA, Wong J, Chu YY, Morrison AC, et al. (2019) The genetic structure of Aedes aegypti populations is driven by boat traffic in the Peruvian Amazon. PLoS Negl Trop Dis 13(9): e0007552. 10.1371/journal.pntd.0007552 [DOI] [PMC free article] [PubMed] [Google Scholar]