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. 2022 Jun 21;27(4):607–614. doi: 10.1007/s12257-022-0044-6

Affinity Peptide-based Electrochemical Biosensor for the Highly Sensitive Detection of Bovine Rotavirus

Chae Hwan Cho 1, Tae Jung Park 2, Jong Pil Park 1,✉
PMCID: PMC9209633  PMID: 35755619

Abstract

Bovine diarrhea is a major concern in the global bovine industry because it can cause significant financial damage. Of the many potential infectious agents that can lead to bovine diarrhea, bovine rotavirus (BRV) is a particular problem due to its high transmissibility and infectivity. Therefore, it is important to prevent the proliferation of BRV using an early detection system. This study developed an affinity peptide-based electrochemical method for use as a rapid detection system for BRV. A BRV-specific peptide was identified via the phage display technique and chemically synthesized. The synthetic peptide was immobilized on a gold electrode through thiol-gold interactions. The performance of the BRV specific binding peptides was evaluated using square wave voltammetry. The developed detection system exhibited a low detection limit (5 copies/mL) and limit of quantitation (2.14 × 102 copies/mL), indicating that it is a promising sensor platform for the monitoring of BRV.

Keywords: bovine diarrhea, bovine rotavirus, phage display, affinity peptide, electrochemical sensor

Acknowledgements

This research was supported by the Chung-Ang University Graduate Research Scholarship, 2021. This study was also supported by the Animal Disease Management Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (120090-2) (T.J. Park).

Ethical Statements

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

Footnotes

Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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