Figure 4.

Myh9 deficiency impairs repopulation capacity of HSC. (A) Schematic diagram of bone marrow competitive reconstitution assay. BM cells (5 × 10⁵) from uninduced RFP^- Myh9^fl/fl:Mx1-cre or littermate control mice were mixed with RFP⁺ wild type mice BM cells (5 × 10⁵) at a 1:1 ratio and transplanted into lethally irradiated RFP^- recipient mice. Six weeks after BMT, recipient mice were treated with three doses of poly I:C to delete Myh9 after hematopoietic reconstitution. The recipient mice were analyzed at 6 weeks after BMT and 16 weeks after poly I:C injection. Bar graphs show the percentage of donor derived RFP^- cells (B), percentage of RFP^-Gr-1⁺ myeloid cells (C), percentage of RFP^-CD19⁺ B cells (D), percentage of RFP^-CD3⁺ T cells (E) in the peripheral blood of recipient mice at 6 weeks after BMT (without poly I:C) and 16 weeks after poly I:C injection (with poly I:C). The percentage of donor derived RFP^- cells (F), RFP^-LSK cells (G), RFP^-Gr-1⁺ myeloid cells (H) in the BM of recipient mice at 16 weeks after poly I:C injection (n = 3–4). All data are shown as mean ± SD. Student t test was used to compare two groups of mice (*** p < 0.001, **** p < 0.0001).