Table 1.
Antibodies for Western blot and Immunofluorescence
| Antibody | Host | Company | Cat. No | Dilution | Duration |
|---|---|---|---|---|---|
| TRPV4 | Rabbit | Abcam | ab39260 | 1:1000 (1:200) | Overnight 4 °C |
| GFAP | Mouse | Cell signaling | #3670 | 1:1000 (1:100) | Overnight 4 °C |
| YAP | Mouse | Protein tech | 66,900–1-Ig | 1:1000 (1:400) | Overnight 4 °C |
| p-YAP(Ser127) | Rabbit | Cell signaling | #13,008 | 1:1000 | Overnight 4 °C |
| STAT3 | Rabbit | Proteintech | 10,253–2-AP | 1:1000 (1:200) | Overnight 4 °C |
| p-STAT3(Tyr705) | Rabbit | Cell signaling | #9145 | 1:2000 | Overnight 4 °C |
| SOCS3 | Rabbit | Proteintech | 14,025–1-AP | 1:1000 (1:200) | Overnight 4 °C |
| IL-6 | Rabbit | Bioss | bs-0782R | 1:1000 | Overnight 4 °C |
| TNF-α | Rabbit | Abclonal | A11534 | 1:1000 | Overnight 4 °C |
| IL-1β | Rabbit | Abcam | ab9722 | 1:1000 | Overnight 4 °C |
| β-Actin | Mouse | Proteintech | HRP-60008 | 1:10,000 | Overnight 4 °C |
| Anti-mouse IgG-HRP | Goat | PMK Biotech | PMK-014-091 M | 1:20,000 | 2 h RT |
| Anti-rabbit IgG-HRP | Goat | PMK Biotech | PMK-014–090 | 1:20,000 | 2 h RT |
|
594 goat anti-mouse IgG 594 goat anti-rabbit IgG 488 goat anti-mouse IgG 488 goat anti-rabbit IgG |
Goat Goat Goat Goat |
Abbkine Abbkine Abbkine Abbkine |
A23410-1 A23420 A23210 A23220 |
1:500 1:500 1:500 1:500 |
2 h RT 2 h RT 2 h RT 2 h RT |
The antibody dilution used for immunofluorescence is enclosed in brackets