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. 2022 Jun 18;14(12):2530. doi: 10.3390/nu14122530

Table 1.

Primer sequences used for qRT-PCR.

Gene (Transcript) Sequences (5′-3′) Products Length/bp
Actb (NM_007393.5) GGCTCCTAGCACCATGAAG 190
CGCAGCTCAGTAACAGTCC
Cpt1a (NM_013495.2) TGACGGCTATGGTGTTTC 124
CATGGCTTGTCTCAAGTG
Ppara (NM_011144.6) TCAAGGCCCGGGTCATACTC 164
CTGGCAGCAGTGGAAGAATC
Acox1 (NM_001377522.1) GCCTGCTGTGTGGGTATGTCATT 150
GTCATGGGCGGGTGCAT
Hmgcs2 (NM_008256.4) GTGTCCCGTCTAATGGAG 180
GAGACACCCAGGATTCAC
Acot3 (NM_001346701.1) TGGTCCTGTGTGGGCTCT 107
TGCCCTCCGAGTGAGTGT
Ehhadh (NM_023737.3) GGAACCCCCACCCGAAAG 129
GATGCGACCCAGTGGCTT
Fgf21 (NM_020013.4) GGTGTCAAAGCCTCTAGGTTTC 139
CATGGGCTTCAGACTGGTACAC
Lipe (NM_010719.5) TCCTGGAACTAAGTGGACGCAAG 93
CAGACACACTCCTGCGCATAGAC
Pnpla2 (NM_001163689.1) AAAGATCGAATTCTAGAGCACC 189
CCACTCCAACAAGCGGA
Pygl (NM_133198.2) CCGGAGACCGTTCTGTGC 104
CTCTACGCCCACGATGCC
Gck (NM_010292.5) CTGGGAGGAACCAACTTCAG 160
CAGAGATGCACTCAGAGATG
Dgat2 (NM_026384.3) GGCTACGTTGGCTGGTAACT 197
TCTTCAGGGTGACTGCGTTC
Slc2a2 (NM_031197.2) AGATTGGGCCAGGTCCAATCC 132
ACTGGAAGCAGAGGGCGATGAC
Slc25a20 (NM_020520.5) GGCTTTGCAGGGATCTTC 142
AGGTGACTCCTTCTTCTC
Acads (NM_007383.3) GTGCTGCCATGTTGAAAG 142
GGCATCTCTGTCACATAC
Acadm (NM_007382.5) GGAGGCTATGGATTCAAC 119
ATGTGCTCACGAGCTATG
Acadl (NM_007381.4) GGTACATGTGGGAGTACC 100
CTTGCGATCAGCTCTTTC
Fasn (NM_007988.3) TAAAGCATGACCTCGTGATGAA 230
GAAGTTCAGTGAGGCGTAGTAG
Scd1 (NM_009127.4) GAACACCCATCCCGAGAGT 176
TGTAAGAACTGGAGATCTCTTGGA
Acaca (NM_133360.2) AGTGATGGTGGCCTGCTCTTG 150
AGCAGACGGTGAGCGCATTA