The protein expression of NLRP3, IL-18, and IL-1β inflammasome in spontaneous miscarriage compared to normal pregnancy. Human primary endometrial stromal cells were transfected with miR-138-5P (25 nM) and si-GPR124 (50 nM) for 48 h. Immunoblot analysis was performed to examine the protein levels of GPR124, IL-1β, IL-18, and NLRP3. (A) The expressions of GPR124, NLRP3, IL-18 and IL-1β protein regulated by miR-138-5p in endometrial stromal cells. (B) The expressions of GPR124, NLRP3, IL-18 and IL-1β protein regulated by si-GPR124 in endometrial stromal cells. miRNA negative control (miR-NC). siRNA control (si-Ctrl). (* p < 0.05, versus control).