Stat4E626G/+ mice have decreased production of IFN-gamma and related cytokines after Cp1038 infection as compared to B6 mice. C57BL/6NJ (black circles), B6-Stat4E626G/+ (red squares) mice were intranasally infected with Cp1038 at the indicated time point after infection mice were sacrificed (N=4–5/time point). Lungs were finely sliced and placed in complete RPMI for 24 hours. Supernatant was then collected and analyzed for IFN-gamma (A), IP-10 (B), and MIG (C) cytokine production by ELISA. Serum was harvested and analyzed for IFN-gamma (D), IP-10 (E), and MIG (F) by ELISA. Significance was determined by Mann-Whitney.