FIG 4.

Target-by-target comparison of differential gene expression in WT PfNF54 and Pfarid^– stage V game oocytes. (A) Graph showing differentially expressed genes (DEGs) from genes predicted to have a role in gametogenesis. Log₂ fold changes are indicated. (B) Acetylated cGMP levels in day 15 gametocyte extracts prepared for WT PfNF54 and Pfarid^–. Bar graph represents means standard errors of the means. Unpaired t test with Welch’s correction was used for statistical analysis. (C) Graph showing DEGs belonging to signaling (kinases, phosphatases, and phospholipases). (D) Graph showing DEGs belonging zinc finger proteins (ZFPs). Log₂ fold changes are indicated.