FIG 1.
Determination of the structure of DndE from N. bangense JCM10635. (A) Size-exclusion chromatography (SEC) determination of DndE. The x axis represents the log value of the protein molecular weight, Kav is calculated by (Ve − Vo)/(Vc − Vo), Ve is the protein elution volume, Vc is the column volume, and Vo is the void volume. Superdex 200 increase 10/300 GL was used for the DndE molecular weight analysis, the column volume is 120 mL, and the void volume is 48.2 mL. The elution volume of DndE is 69 mL; thus, the molecular weight based on SEC is 16.8 kDa, which is closed to the monomer molecular weight of 15.3 kDa. (B) Crystals and diffraction image of DndE. (C) The dndCDEA locus and crystal structure of DndE shown in cartoon mode.