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. 2022 Jun 29;81:104132. doi: 10.1016/j.ebiom.2022.104132

Figure 3.

Figure 3

(A) TEER value of monolayers in transwell membrane tested at 3, 5, 10, 13, 16, 20, 23 and 25 days after seeding. (B) Schematic representation of virus inoculation in organoid cells in transwell system. (C) Quantification of released viruses from monolayers inoculated with 229E particles. (D) Quantification of released viruses from monolayers inoculated with OC43 particles. (E) to (G) Immunofluorescence staining for viral dsRNA and β-tubulin (ciliated cell marker) in transwell monolayers at 72 h post-inoculation (Scale bar = 25 μm).