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. 2022 Jun 29;81:104132. doi: 10.1016/j.ebiom.2022.104132

Figure 4.

Figure 4

Kinetics of 229E and OC43 virus replication and production at different temperatures. (A) 229E replication kinetics in undifferentiated hAOs at 33 °C and 37 °C at 1 h, 1 day, 3 day, 5 day, 7 day post-inoculation (n=4). (B) The kinetics of 229E virus production (n=4). (C) The kinetics of OC43 viral replication (n=3–4). (D) The kinetics of OC43 virus production (n=3–4). (E) Immunofluorescence staining of 229E viral dsRNA and EpCAM (epithelial membrane marker) in undifferentiated hAOs at different temperatures at 3 days post-inoculation. (F) Immunofluorescence staining of OC43 viral dsRNA and EpCAM in undifferentiated hAOs. The kinetics of 229E viral replication (G) and production (H) in differentiated hAOs at 33 °C and 37 °C at 1 h, 1 day, 3 days, 5 days, 7 days post-inoculation (n=2–3). The kinetics of OC43 replication (I) and production (J) in differentiated hAOs (n=2–3). (K) and (L) Immunofluorescence staining of viral dsRNA and EpCAM in differentiated hAOs at 33 °C and 37 °C at 3 days post-inoculation. Scale bar = 25 μm.