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. 2022 May 2;7(3):e00009-22. doi: 10.1128/msphere.00009-22

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FIG 2 — Relative percent T. pallidum Nichols DNA in total DNA for nonenriched and NEB+MDA- and SWGA-enriched spiked samples. Spiked samples were composed of a 10-fold dilution series of T. pallidum Nichols DNA and a constant concentration of human DNA. The percent T. pallidum DNA in total DNA was calculated based on the input DNA concentration and gDNA copies/μL (nonenriched) and the DNA concentration and gDNA copies/μL for the Nichols-spiked samples postenrichment (NEB+MDA or SWGA). Genome copies were estimated from measured T. pallidum polA copies/μL of DNA extract. The y axis is log₁₀-scaled for depiction of the nonenriched dilution series. Error bars represent the standard error among three replicate samples.