Skip to main content
. 2022 Jul 3;25(8):104709. doi: 10.1016/j.isci.2022.104709
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-CoV-2 S2 (1A9) Genetex, Hsinchu, Taiwan GTX632604
Anti-VSV-M Absolute, Boston, MA Ab01404–2.0
Anti-beta-actin Genetex, Hsinchu, Taiwan GTX109639
Anti-CoV-2 Spike S1 Genetex, Hsinchu, Taiwan GTX635672
Anti-CoV-2 mature S2 (ECD 45) Antaimmu BioMed, Hsinchu, Taiwan ATBM-A-08
Anti-PDI Cell signaling, Danvers, MA 45596
Anti-RCAS1 Cell signaling, Danvers, MA 12290

Bacterial and virus strains

DH5α Yeastern Biotech, Taipei, Taiwan FYE678
SARS-CoV-2 Taiwan Centers for Disease Control N/A

Chemicals, peptides, and recombinant proteins

PNGase F NEB, Ipswich, MA P0704S
Lipofectamine 2000 ThermoFisher, Waltham, MA 11668
DAPI ThermoFisher, Waltham, MA D21490
2-Bromohexadecanoic acid (2BP) Sigma-Aldrich, Darmstadt, Germany 238422
M199 medium Gibco; ThermoFisher, Waltham, MA 11150–059
TPCK-trypsin Sigma-Aldrich, Darmstadt, Germany T8802
MTT solution Sigma-Aldrich, Darmstadt, Germany M-0283
Versene solution Gibco, Waltham, MA 15040066
Protease inhibitor cocktail ThermoFisher, Waltham, MA 78437
Phenylmethylsulfonyl fluoride Sigma-Aldrich, Darmstadt, Germany 329-98-6
Transcription Factor Buffer Set BD Pharmingen™; ThermoFisher, Waltham, MA AB_2869424
SuperNuclease SinoBiological,Beijing,China SSNP01
TCEP solution ThermoFisher, Waltham, MA 77720
N-Ethylmaleimide Sigma-Aldrich, Darmstadt, Germany 04259
mPEG-Mal Sigma-Aldrich, Darmstadt, Germany 99126-64-4
MEM alpha medium Cytiva; Hyclone, Marlborough, MA SH30265
FBS Cytiva; Hyclone, Marlborough, MA SH30396
Pen Strep solution Corning, New York 20-003
HEPES buffer Biological Industries, Israel 03-025
DMEM/High glucose medium Cytiva; Hyclone, Marlborough, MA SH30243
MEM medium ThermoFisher, Waltham, MA 11095080

Experimental models: Cell lines

Vero E6 provided by Dr. Shiow-Ju Lee, NHRI N/A
HEK293T/17 provided by Dr. Chia-Yi Yu, NHRI N/A
BHK21 provided by Dr. Chia-Yi Yu, NHRI N/A
Calu-3 provided by Dr. Yueh-Hsin Ping, NYCU N/A

Software and algorithms

GraphPad PRISM 6.2 https://www.graphpad.com N/A
ImageJ https://imagej.nih.gov/ij/ N/A