Antibodies |
|
Anti-CoV-2 S2 (1A9) |
Genetex, Hsinchu, Taiwan |
GTX632604 |
Anti-VSV-M |
Absolute, Boston, MA |
Ab01404–2.0 |
Anti-beta-actin |
Genetex, Hsinchu, Taiwan |
GTX109639 |
Anti-CoV-2 Spike S1 |
Genetex, Hsinchu, Taiwan |
GTX635672 |
Anti-CoV-2 mature S2 (ECD 45) |
Antaimmu BioMed, Hsinchu, Taiwan |
ATBM-A-08 |
Anti-PDI |
Cell signaling, Danvers, MA |
45596 |
Anti-RCAS1 |
Cell signaling, Danvers, MA |
12290 |
|
Bacterial and virus strains |
|
DH5α |
Yeastern Biotech, Taipei, Taiwan |
FYE678 |
SARS-CoV-2 |
Taiwan Centers for Disease Control |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
PNGase F |
NEB, Ipswich, MA |
P0704S |
Lipofectamine 2000 |
ThermoFisher, Waltham, MA |
11668 |
DAPI |
ThermoFisher, Waltham, MA |
D21490 |
2-Bromohexadecanoic acid (2BP) |
Sigma-Aldrich, Darmstadt, Germany |
238422 |
M199 medium |
Gibco; ThermoFisher, Waltham, MA |
11150–059 |
TPCK-trypsin |
Sigma-Aldrich, Darmstadt, Germany |
T8802 |
MTT solution |
Sigma-Aldrich, Darmstadt, Germany |
M-0283 |
Versene solution |
Gibco, Waltham, MA |
15040066 |
Protease inhibitor cocktail |
ThermoFisher, Waltham, MA |
78437 |
Phenylmethylsulfonyl fluoride |
Sigma-Aldrich, Darmstadt, Germany |
329-98-6 |
Transcription Factor Buffer Set |
BD Pharmingen™; ThermoFisher, Waltham, MA |
AB_2869424 |
SuperNuclease |
SinoBiological,Beijing,China |
SSNP01 |
TCEP solution |
ThermoFisher, Waltham, MA |
77720 |
N-Ethylmaleimide |
Sigma-Aldrich, Darmstadt, Germany |
04259 |
mPEG-Mal |
Sigma-Aldrich, Darmstadt, Germany |
99126-64-4 |
MEM alpha medium |
Cytiva; Hyclone, Marlborough, MA |
SH30265 |
FBS |
Cytiva; Hyclone, Marlborough, MA |
SH30396 |
Pen Strep solution |
Corning, New York |
20-003 |
HEPES buffer |
Biological Industries, Israel |
03-025 |
DMEM/High glucose medium |
Cytiva; Hyclone, Marlborough, MA |
SH30243 |
MEM medium |
ThermoFisher, Waltham, MA |
11095080 |
|
Experimental models: Cell lines |
|
Vero E6 |
provided by Dr. Shiow-Ju Lee, NHRI |
N/A |
HEK293T/17 |
provided by Dr. Chia-Yi Yu, NHRI |
N/A |
BHK21 |
provided by Dr. Chia-Yi Yu, NHRI |
N/A |
Calu-3 |
provided by Dr. Yueh-Hsin Ping, NYCU |
N/A |
|
Software and algorithms |
|
GraphPad PRISM 6.2 |
https://www.graphpad.com |
N/A |
ImageJ |
https://imagej.nih.gov/ij/ |
N/A |