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. 2022 Jun 29;25(7):104662. doi: 10.1016/j.isci.2022.104662

General method to stabilize mesophilic proteins in hyperthermal water

Xiaoqian Xin, Youwei Xu, Hu Shi, Xiaowen Liu
PMCID: PMC9254118  PMID: 35800752

Main Text

(iScience 24, 102503; May 21, 2021)

As a result of an author oversight, in the originally published version of this article, T in the equation ΔGD = -RTlnKD was mistakenly substituted in the form of Fahrenheit temperature when calculating the ΔGD, which should have used Kelvin temperature. This lead biased Tm values in the subsequent calculations. Therefore, the main errors appear in the figures related to ΔGD and the manuscript and tables related to Tm. The authors have now replaced the wrong figure with the correct one in Figures 4B, 4D, and 4F and Figure S10. The data in Table S9 and Table S10 have also been corrected. These errors have now been corrected in the article online. All the authors agree that the changes have no impact on the outcome or conclusions of the study. The authors apologize for any confusion caused to the readers.

Figure 4.

Figure 4

Thermal protection effect of P1M5 offering with other mesophilic proteins (corrected)

Figure 4.

Figure 4

Thermal protection effect of P1M5 offering with other mesophilic proteins (original)

Figure S10.

Figure S10

Plots of free denaturation energy (ΔGD) of BSA against temperature, related to Figure 3. (corrected)

Figure S10.

Figure S10

Plots of free denaturation energy (ΔGD) of BSA against temperature, related to Figure 3. (original)

Table S9.

Thermodynamic behaviour of BSA and PxMy@BSA in hyperthermal water, Related to Figure 3. (corrected)

Tm (°C) ΔSm (J·K-1 ·mol-1) ΔHm (kJ ·mol-1)
BSA 65.9 1173.6 77.3
P2M1@BSA 64.8 460.9 29.9
P1M1@BSA 80.0 419.3 33.5
P1M2@BSA 107.0 210.2 22.5
P1M5@BSA 130.6 144.1 18.8
P1M10@BSA 124.5 145.5 18.1

Table S9.

Thermodynamic behaviour of BSA and PxMy@BSA in hyperthermal water, Related to Figure 3. (original)

Tm (°C) ΔSm (J·K-1 ·mol-1) ΔHm (kJ ·mol-1)
BSA 65.4 511.4 33.5
P2M1@BSA 62.3 167.7 10.5
P1M1@BSA 77.5 163.8 12.7
P1M2@BSA 127.1 55.5 7.1
P1M5@BSA 181.6 33.9 6.2
P1M10@BSA 171.5 33.3 5.7

Table S10.

Thermodynamic behaviour of TRF, MB and INS with or without P1M5 encapsulation in hyperthermal water, Related to Figure 4. (corrected)

Tm (°C) ΔSm (J·K-1mol-1) ΔHm (kJ·mol-1)
TRF 80.7 533.2 43.0
P1M5@TRF 167.0 79.4 13.3
MB 93.6 472.8 44.2
P1M5@MB 97.9 192.3 18.8
INS 115.8 104.4 12.1
P1M5@INS 153.8 100.7 15.5

Table S10.

Thermodynamic behaviour of TRF, MB and INS with or without P1M5 encapsulation in hyperthermal water, Related to Figure 4. (original)

Tm (°C) ΔSm (JK-1mol-1) ΔHm (kJ·mol-1)
TRF 78.4 238.7 18.7
P1M5@TRF 326.6 13.7 4.5
MB 87.6 210.9 18.4
P1M5@MB 99.5 70.5 7.0
INS 132.3 32.4 4.3
P1M5@INS 237.2 23.1 5.5

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