Figure 6. Cartoon model of reconstituted kinetochore dynamics on the microtubule Yeast kinetochores reconstituted binding with individual yeast microtubules in a yeast protein extract.

These laterally-bound kinetochores travel in a directional manner to the plus end of the microtubule, powered by the kinesin-8, Kip3. Upon reaching the end of the microtubule, the kinetochore transitions to end-on attachment. Establishment of this end-on attachment coincides with onset of microtubule depolymerization and the end-bound kinetochore preventing the microtubule from elongating again.