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. 2022 Jun 28;11(13):2046. doi: 10.3390/cells11132046

Figure 2.

Figure 2

Plasma cell ultrastructure and glycocalyx thickness in the jejunal mucosa of HC subjects and IBS−D patients. (A) Representative transmission electron microscopy images of plasma cells from HC (left panel) and IBS−D (right panel) groups. Inserts show the morphology of the RER in each group. Bar represents 1 µm. (B) Quantification of the percentage of RER area in the cytoplasm (%RER) in plasma cells in HC (n = 11) and IBS−D (n = 9) groups. (C) Correlation assessment between the %RER and the number of mitochondria per plasma cell (pooled participants, n = 20). (D) Representative transmission electron microscopy images of the glycocalyx overlying the microvilli from HC (left panel) and IBS-D (right panel) groups. Bar represents 200 nm; dashed square height indicates 260 nm. (E) Quantification of the glycocalyx thickness in HC (n = 9) and IBS−D (n = 10) groups. (F) Correlation assessment between the %RER and the glycocalyx thickness (pooled participants, n = 16). IBS−D: diarrhoea−predominant irritable bowel syndrome; HC: healthy control; PC: plasma cell. Mann−Whitney U test was used for comparisons between groups and Pearson’s correlation coefficient analysis for correlations between the parameters. Differences were considered significant when p ≤ 0.05.