Table 3. Frequencies and single nucleotide polymorphism (SNP) of G6PD gene and association analysis with susceptibility to severe malaria (SM).

G6PD-SNP	NCBI dbSNP number	Phénotype	MAF				HWE
			SM	UM	CTR	Overall Population	SM vs UM		UM vs CTR	SM vs CTR
							P-value	OR (95% IC)	P-value	P-value
+10588_A >G	rs762515		0.280	0.373	0.325	0.329	0.047	0.65 (0.43–0.99)	0.344	0.415
+10707_G >A	newSNP		0	0.004	0	0.001	1		1	1
+10776_G >C	newSNP		0	0.008	0	0.003	0.509		0.517	1
+10869_G >A	rs1050828	G6PD202A	0.022	0.032	0.018	0.026	0.568		0.536	1
+10983_G >C	newSNP		0.005	0.008	0.012	0.008	1		1	0.607
+11000_C >T	rs782500951		0.005	0.004	0	0.003	1		1	1

Notes.

The p values for statistical tests were performed using the linear regression model analysis for each polymorphisms. Association analysis G6PD for polymorphisms (Table 3) and HBB polymorphisms (Table 4) were performed separately, and corrections to each other were applied to reflect the real effect of each. Analysis has been carried out by comparing SM vs UM, UM vs CTR, and SM vs CTR. Borderline (0.05 ≤ p ≤ 0.1) and significant (0 −  ≤ p ≤ 0.05) p values are in bold. The OR (odds ratio) and CI (Confidence intervals) were shown when p values were significant. The phenotype attributed by from each polymorphism was mentioned (from NCBI) as: Erythrocytosis 6 familial (E6 familial), Beta-thalassemia (β-thalassemia), Fetal hemoglobin quantitative trait loci 1 (Fetal HQTL-1), Heinz body anaemia (H-B-A), METHEMOGLOBINEMIA (MTH). The SNP labelled as “benign” were ‘non-affected SNP’, then non associated with many diseases such as Thalassemia, HbSS disease, and/or any other pathology with a notorious phenotype.

SM

Severe Malaria

UM

Uncomplicated Malaria

CTR

Control group

MAF

Minor Allele Frequency

HWE

Hardy–Weinberg