Figure 6.

Effect of agerarin on the suppression of IL-4-induced PER2 expression in HaCaT cells synchronized with Dex. HaCaT cells were synchronized with 100 nM Dex for 2 h, followed by the addition of in the presence (+) or absence (−) of 20 ng/mL IL-4 or 20 ng/mL IL-4 plus 40 μM agerarin. After 24 h, whole-cell lysates were prepared and immunoblotted using anti-PER2 antibodies. GAPDH was used as the loading control. Band intensities of PER2 protein were measured using ImageJ and normalized to GAPDH. Dex, dexamethasone.