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. Author manuscript; available in PMC: 2022 Jul 11.
Published in final edited form as: Pain. 2021 Mar 1;162(3):895–906. doi: 10.1097/j.pain.0000000000002092

Figure 2. D2 receptor signaling modulated state-dependent LTD at PL-cNAc d2SPN synapses.

Figure 2.

a-b, Representative images showing Chronos-GFP (green) expression in PL-PFC and BLA at 4-week post injection (scale bar: 0.5 mm). c-d, Bath application of CB1 receptor agonist WIN 55,212–2 induced LTD of AMPAR EPSC amplitude in PL-cNAc d2SPN synapses, but had no effect on BLA-d2SPN synapses (n = 5 neurons from 5 mice per group; Wilcoxon test with W = −4543, p < 0.0001; calibration for c: 50 pA, 10 ms). e, Diagram of D2R-dependence of the eCb-dependent LTD. f, The representative recording traces showing the EPSCs of PL-cNAc d2SPNs synapses before and after state-dependent LTD induction in the presence of D2 receptor agonist quinpirole (10 μM, calibration: 50 pA, 10 ms). g, State-dependent LTD induction protocol (calibration: 300 pA, 500 ms). h, At PL-cNAc d2SPNs synapses, the induction protocol elicited a robust LTD in the presence of quinpirole but not sulpiride (10 μM of quinpirole and sulpiride, n = 5 neurons from 5 mice per group; Wilcoxon test with W = −5130, p < 0.0001). Data are presented as median with shaded interquartile (quartile 1 to quartile 3).