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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Cytotherapy. 2021 Apr 20;23(8):704–714. doi: 10.1016/j.jcyt.2021.02.118

Table I:

Global summary of CD4 Teff and tTreg Data

Cell type Stim# Rapa Fold Exp. (Approx.) Hierarchical clustering TF WGCNA Overall conclusions
Module Representative genes Module Representative TF
CD4 Teff 1 or 2 NA 400–40,000 Green IL-7RA, Themis, TBX21, ID2, CD40L Eomes, TOX; multiple inhibitory receptors and cytokines Green Tbx21, Eomes, BATF

  • T effector/memory gene expression

  • TOX and inhibitory receptor expression suggest later stage of differentiation.
tTreg 1 or 2 − 100–1000 Red Foxp3, Helios, UTS2, SLC14A1, CDKN2B, Eos, PLCL1, SOCS2, CD80, GPR55, LSR Violet Foxp3, Helios, Eos, Satbl.Lefl, Bach2, TCF1

  • Maintain Treg signature

  • Treg locking gene expression similar to resting tTreg
Lilac RTKN2, CASK, SELP, MY05C, CD27, DGKA, and CCR6
tTreg 1 or 2 + 50–2500 Red Foxp3, Helios, UTS2, SLC14A1, CDKN2B, Eos, PLCL1, SOCS2, CD80, GPR55, LSR Violet Foxp3, Helios, Eos, Satbl, Lefl, Bach2, TCF1

  • Maintain Treg signature

  • Treg locking gene expression similar to resting tTreg
Lilac RTKN2, CASK, SELP, MY05C, CD27, DGKA, and CCR6
tTreg 3 or 5 + 40,000–10,000,000 Red Foxp3, Helios, UTS2, SLC14A1, CDKN2B, Eos, PLCL1, SOCS2, CD80, GPR55, LSR Yellow IRF4, GATA1, PRDM1

  • Maintain Treg signature

  • Treg locking gene expression similar to activated tTreg

  • May be a precursor to Tfr pool.
Purple RTKN2, CASK, SELP, MY05C, CD27, DGKA, and CCR6