Abstract
In experiments on narcotized male rats (n=85), the mean electroimpedance Z and peak-to-peak magnitudes (the swing ranges) of passive (ΔZp) and active (ΔZa) pulsatile electroimpedance oscillations of isolated segment of femoral arteries were determined in situ. These rheographic parameters (RP) were measured in intact animals and in those with modeled chronic myocardial infarction, chronic denervation of the right hind leg, as well as in rats subjected to sham operations to mimic denervation or infarction (with thoracic trauma). The rats with modeled myocardial infarction demonstrated decreasing trends of all RP. In sham-operated rats with thoracic trauma, ΔZp increased significantly on postsurgery months 2-4 by 4.3 times in comparison with the control. No essential correlation was found in denervated rats between RP of any femoral artery and severity of neuropathic pain syndrome assessed by autotomy of the operated leg. In these rats, the mean electroimpedance Z of any femoral artery was significantly greater than the control level. They demonstrated especially high values of ΔZp with significant difference between ΔZp of innervated and denervated hind leg. In denervated rats, ΔZa was significantly greater than the control value without significant difference between ΔZa of both femoral arteries. The paradoxically great increase of ΔZp (100- and 50-fold for innervated and denervated legs, respectively) and a significant 3-fold increment of ΔZa in both hind legs provoked by denervation of one of them are discussed in relation to searching for the ways of systemic influences on vascular network in clinics and experiments.
Key Words: electroangiology, rat femoral arteries, active and passive pulsing, denervation, myocardial infarction
Footnotes
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 3, pp. 294-300, March, 2022
References
- 1.Karpova MN, Kuznetsova LV, Klishina NY, Kukushkin ML. Effect of ipidacrine on acute pain and development of neuropathic pain syndrome in Wistar rats. Patol. Fiziol. Eksper. Ter. 2019;63(2):29–33. doi: 10.25557/0031-2991.2019.02.29-33. [DOI] [Google Scholar]
- 2.Kedrov AA. A new method for determining the pulse fluctuations of blood volume in vessels of various parts of the human body. Klin. Med. 1941;19(1):71–80. [Google Scholar]
- 3.Kryzhanovskii NG, Reshetnyak VK, Kukushkin ML, Gorizontova MP, Smirnova VS. Pain syndrome in rats after injury to the sciatic nerve. Patol. Fiziol. Eksper. Ter. 1991;6:8–9. [PubMed] [Google Scholar]
- 4.Kryzhanovskii SA, Tsorin IB, Ionova EO, Stolyaruk VN, Vititnova MB, Barchukov VV, Miroshkina IA, Sorokina AV, Kozhevnikova LM, Durnev AD. A translational model of chronic heart failure in rats. Patol. Fiziol. Eksper. Ter. 2018;62(2):136–148. doi: 10.1007/s10517-019-04604-y. [DOI] [PubMed] [Google Scholar]
- 5.Kukushkin ML, Smirnova VS. Significance of the functional state of the sciatic nerve at the time of its transection for subsequent development of pain syndrome in rats. Patol. Fiziol. Eksper. Ter. 1993;37(1):7–8. [PubMed] [Google Scholar]
- 6.Markosyan TG, Alyaev YG, Glybochko PV, Kirpatovsky VI, Mudraya IS, Nikitin SS, Popov EG, Revenko SV. Rhythmic oscillations of human penile bioimpedance in healthy individuals and in patients with vascular erectile dysfunction. Bull. Exp. Biol. Med. 2011;151(4):405–410. doi: 10.1007/s10517-011-1342-8. [DOI] [PubMed] [Google Scholar]
- 7.Glybochko PV, Aljaev JG, Grigorjan VA, Markosjan TG, Revenko SV, Nikitin SS, Mudraja IS, Limonov EV. Patent RU No. 2444989. Method of diagnosing vasculogenic erectile dysfunction. Byull. No. 8. Published March 20, 2012.
- 8.Revenko SV, Tikhomirova LN, Gavrilov IY, Tarakanov IA. Effect of alternating electric current on pulsation mode of rat major arteries in situ. Bull. Exp. Biol. Med. 2019;167(3):305–310. doi: 10.1007/s10517-019-04515-y. [DOI] [PubMed] [Google Scholar]
- 9.Revenko SV, Tikhomirova LN, Nesterov AV, Tarakanov IA. Bimodal electrical properties of rat major artery segment in situ. Bull. Exp. Biol. Med. 2018;164(6):701–706. doi: 10.1007/s10517-018-4062-5. [DOI] [PubMed] [Google Scholar]
- 10.Tarakanov IA, Tikhomirova LN, Gavrilov IY, Revenko SV. Relationship between passive and active pulsations of rat artery as vascular extension of Frank—Starling law. Bull. Exp. Biol. Med. 2021;170(6):719–723. doi: 10.1007/s10517-021-05140-4. [DOI] [PubMed] [Google Scholar]
- 11.Alphonsus CS, Rodseth RN. The endothelial glycocalyx: a review of the vascular barrier. Anaesthesia. 2014;69(7):777–784. doi: 10.1111/anae.12661. [DOI] [PubMed] [Google Scholar]
- 12.Belousov YM, Krainov VP, Revenko SV. Hydrodynamic model of blood flow in major arteries pulsing in various modes. Med. Biol. Eng. Comput. 2021;59(9):1785–1794. doi: 10.1007/s11517-021-02413-y. [DOI] [PubMed] [Google Scholar]
- 13.Christensen T, Jørgensen J, Neubauer B. Diameter and pulsatile oscillations of the femoral artery recorded by ultrasound. Acta Radiol. Diagn. (Stockh). 1984;25(4):313–316. doi: 10.1177/028418518402500411. [DOI] [PubMed] [Google Scholar]
- 14.Stamatelopoulos K, Georgiopoulos G, Baker KF, Tiseo G, Delialis D, Lazaridis C, Barbieri G, Masi S, Vlachogiannis NI, Sopova K, Mengozzi A, Ghiadoni L, van der Loeff IS, Hanrath AT, Ajdini B, Vlachopoulos C, Dimopoulos MA, Duncan CJA, Falcone M, Stellos K. Pisa COVID-19 Research Group; Newcastle COVID-19 Research Group. Estimated pulse wave velocity improves risk stratification for all-cause mortality in patients with COVID-19. Sci. Rep. 2021;11(1):20239. doi: 10.1038/s41598-021-99050-0. [DOI] [PMC free article] [PubMed] [Google Scholar]