Author correction to ‘Ruscogenin alleviates LPS-triggered pulmonary endothelial barrier dysfunction through targeting NMMHC IIA to modulate TLR4 signaling’ [Acta Pharmaceutica Sinica B 12 (2022) 1198–1212]

The authors regret that there were some errors or inexact descriptions in author affiliation, the section of materials and methods, and the figure caption of Fig. 2A owing to the negligence of authors when writing the manuscript, and there was a picture error in Fig. 10 and graphical abstract figure owing to the inappropriate selection for the picture of Radix Ophiopogon japonicus. In author affiliation, “Department of Pharmacology of Chinese Material Medica” should be corrected to “Department of Pharmacology of Chinese Materia Medica”. In 2.4. Evans blue albumin (EBA) pulmonary transvascular flux measurement, “After 2 h, mice were anesthetized and intravascular EBA perfused with saline through the right ventricle for 5 min”, intravascular EBA should be deleted due to grammar error. In 2.11. Serial affinity chromatography, “In brief, lysate from MLECs (1 mL) was shaken mildly with RUS affinity resin…”, MLECs should be corrected to ECV304 cells, TNF-α-activated ECV304 cells, and HUVECs. And in “The samples were subjected to sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and the resulting bands were stained with silver staining and then comparatively analyzed to identify specific binding proteins”, silver staining should be corrected to Coomassie blue staining. In figure caption (Fig. 2A), “A1 and A2 represent samples from ECV304 cells, B1 and B2 represent samples from TNF-α-activated ECV304 cells, C1 and C2 represent samples from HUVECs” should be added to the end of Fig. 2A caption. In Fig. 10 and graphical abstract figure, the picture of Radix Ophiopogon japonicus should be changed to the present revised photo taken by one of the authors instead of the previous picture download from internet. The authors revise 2.4. Evans blue albumin (EBA) pulmonary transvascular flux measurement, 2.11. Serial affinity chromatography, Fig. 2A caption, and the picture of Radix Ophiopogon japonicus in Fig. 10 and graphical abstract figure as bellow. The revisions do not affect any results and conclusions of the current work.

The authors apologize for any inconvenience caused to the journal and readers.

Section 2.4. Evans blue albumin (EBA) pulmonary transvascular flux measurement is revised as:

After LPS treatment of mice via intratracheal instillation (5 mg/kg) for 4 h, EBA dye (50 mg/kg) was administered via tail vein injection. After 2 h, mice were anesthetized and perfused with saline through the right ventricle for 5 min. Mouse lungs were excised, homogenized in 1 mL phosphate buffer solution (PBS), and extracted in 2 mL formamide for 18 h at 60 °C. The EBA concentration was determined based on OD₆₂₀ and OD₇₄₀ values.

Section 2.11. Serial affinity chromatography is revised as:

Serial affinity chromatography with some modifications was used to separate and identify the target proteins of RUS as described previously^19,20. In brief, lysates from ECV304 cells, TNF-α-activated ECV304 cells, and HUVECs (1 mL) were shaken mildly with RUS affinity resin (30 μL) (A1, B1, and C1) for 1.5 h at 4 °C, then centrifuged at 16,000 ×g for 1 min. And the supernatants were mixed with another RUS affinity resin (30 μL) (A2, B2, and C2) at 4 °C, again for 1.5 h. Then the resulting resin was washed 3 times with 1 mL lysate buffer, resuspended in 30 μL of 2× loading buffer and heated at 95 °C for 5 min. The samples were subjected to sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and the resulting bands were stained with Coomassie blue staining and then comparatively analyzed to identify specific binding proteins.

Figure caption of Fig. 2A is revised as:

(A) Serial affinity chromatography was used to capture the specific binding proteins of RUS and NMMHC IIA was identified as a RUS targeting protein. A comparison of proteins bound to the first resins (A1, B1, and C1) and second resins (A2, B2, and C2) showed that only the protein marked by arrow decreased significantly in amount, indicating that it was a specific binding protein. M is for the protein marker. A1 and A2 represent samples from ECV304 cells, B1 and B2 represent samples from TNF-α-activated ECV304 cells, C1 and C2 represent samples from HUVECs.

The picture of Radix Ophiopogon japonicus in Fig. 10 and graphical abstract figure is revised as: