An official website of the United States government
Here's how you know
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
As a library, NLM provides access to scientific literature. Inclusion in an NLM database does not imply endorsement of, or agreement with,
the contents by NLM or the National Institutes of Health.
Learn more:
PMC Disclaimer
|
PMC Copyright Notice
4 Department of International cooperation, National Medical Research Center of Rehabilitation and Balneology, Ministry of Healthcare of Russian Federation
1 Interdepartmental Research Centre of Myology, Department of Biomedical Sciences, University of Padua, Italy
2
Armando & Carmela Mioni-Carraro Foundation for Translational Myology, Padua, Italy
3 Department of Sports and Health Sciences, Section Regulative and Preventive Physiology, University of Potsdam, Germany
4 Department of International cooperation, National Medical Research Center of Rehabilitation and Balneology, Ministry of Healthcare of Russian Federation
5 Orthopaedic Institution, Landspitali University Hospital, Reykjavik, Iceland
6
Ludwig Boltzmann Institute for Rehabilitation Research, St Pölten, Austria
7
Institute of Physical Medicine and Rehabilitation, Physiko und Rheumatherapie, St. Pölten, Austria
8 Department of Aging and Geriatric Research, Division of Biology of Aging, College of Medicine, University of Florida, FL, USA
9 Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria
10 Institute of Biological Sciences, University of Leeds, UK
11 Centre of Research in Myology, Institute of Myology, Sorbonne Université, INSERM, Paris, France
12 Department of Surgery, Oncology and Gastroenterology (DISCOG), University of Padua, Italy
✉
Department of Biomedical Sciences, University of Padua, Italy. ORCID iD: 0000-0002-0924-4998 ugo.carraro@unipd.it
E-publishing is supported in part by the Armando & Carmela Mioni-Carraro Foundation for Translational Myology Padua, Italy and by PAGEpress, Pavia, Italy.
Received 2022 Apr 12; Accepted 2022 Apr 12; Collection date 2022 Jul 8.
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Despite COVID-19 outbreak, the program of the 2022 Padua Days of Muscle and Mobility Medicine (PDM3) was confirmed On-site in February from March 30 to April 2, 2022 to be held at the University of Padua Aula Magna and at Conference Hall of the Hotel Petrarca of Thermae of Euganean Hills (Padua), Italy. Over 130 abstracts, including the last-minute submissions listed below, convinced organizers to extend the program to five days. The sponsorship of the University of Florida and the willingness of attendees to meet friends after two years of virtual conferences were the keys of success, despite concerns for current events in East Europe. Only fourteen Virtual presentations were in the final program, eight due to last-minute Coronavirus infections and six for East Europe problems. The first two days of the programincluded scientists and clinicians of the University of Florida, USA and their invitees from Canada, France, Italy, Swiden, Swiss, UK and USA. Researchers and clinicians from Austria, Belgium, France, Germany, Iceland, Ireland, Italy, Russia, Slovakia, Slovenia, UK and USA filled the program of last three days more oriented to aging and rehabilitation. The large majority of abstracts was e-published before the meeting; here are last-minute abstracts and the final program. The program of the 2023 On-site PDM3 was informally designed during the Meeting, but will be circulated during 2022 summer. Fix the dates in your agenda from Thursday March 28 to Friday March 31. For now, please, submit Communications to the European Journal of Translational Myology, PAGEpress, Pavia, Italy and Original Articles or Reviews to the Journal Diagnostics, MDPI, Basel, Swiss. Both journals will host Special PDM3 Sections and will apply 50% discount on editorial processing fees to the first 15 accepted typescripts.
Key Words: Translational Myology and Mobility Medicine, Padua Days of Muscle & Mobility Medicine (PDM3 On-site), last minute program and abstracts
Ethical Publication Statement
We confirm that we have read the Journal’s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines.
Meetings on muscle biology, physiology, medicine and rehabilitation, called Padua Muscle Days (PMDs), started 30 years ago, as a series of two days Seminars, specifically to provide advice on Basic and Applied Myology (BAM). From 2010 when the Journal BAM was renamed European Journal of Translational Myology (EJTM) theMeeting was renamed Padua Days of Muscle and Mobility Medicine (PDM3). Always the interest was on implementing basic research and clinical trials to helping prevent, manage and rehabilitate girls, boys, young adults and elderly persons suffering with mobility disorders. In the autumn of 2021, the 2022 Padua Days of Muscle and Mobility Medicine (PDM3) were planned to be held from March 30 to April 2, 2022. Despite the COVID-19 outbreak continued to impose restrictions, the meeting was confirmed as an on-site event in late February, 2022. Meantime, over 130 abstracts, including the last-minute submissions here included, were submitted and convinced organizers to extend the program to five days to provide time slots to young PhD trainees and post-docs. The willingness of attendees to meet friends after two years of virtual conferences is the key of success. Despite concerns of recent events in East Europe only 13 Virtual presentations are listed in the final program (see below), 6 of them due to last-minute Coronavirus infections of the Speakers. The day one and two of the final programincluded scientists and clinicians of the University of Florida, USA and their invitees from Canada, France, Italy, Sweden, Swiss, U.K, and USA. Researchers and clinicians from Austria, Belgium, France, Germany, Iceland, Ireland, Italy, Russia, Slovakia, Slovenia, UK and USA filled the program of the last three days more oriented to Aging and Rehabilitation. The abstracts collected up to February 28, 2022 were e-published in the European Journal of Translational Myology (EJTM), 32(1) 2022 (Link to: https://www.pagepressjournals.org/index.php/bam/article/view/10440), together with the program of the 2022 On-site PDM3 organized in the Aula Magna of the University of Padua (March 30) and for March 31 - April 3 at the Conference Hall of the Hotel Petrarca, Thermae of Euganean Hills (Padua), Italy. Indeed, 130 abstracts, including the last-minute submissions listed below, convinced organizers to extend the program to five days. The Program ended on late morning of Sunday April 3, 2022 with invitation to join the 2023 PDM3, at the Thermae of Euganean Hills (Padua), Italy. During fruitful informal after-dinner discussions among Potential Organizers the Program of the 2023 On-site PDM3 was almost designed, but it will be circulated in summer 2022. Anyhow, you are invited to fix the dates in your agenda from Thursday March 28 to Friday March 31, 2023. In this report, beside the 2022 On-site PDM3 program and the last-minute abstracts, the Emails of all attendees are listed, together with some of the messages that were sent to thank the local organizers. For now, please, submit Communications to the European Journal of Translational Myology, PAGEpress, Pavia, Italy (Link to:https://pagepressjournals.org/index.php/bam/about/submissions) and Original Articles or Reviews to the Journal Diagnostics, MDPI, Basel, Swiss (Link to: https://www.mdpi.com/journal/diagnostics/instructions). Both journals will host PDM3 Special Sections and will provide 50% discount on the editorial processing fees to the first 15 accepted typescripts.
We invite all EJTM authors and readers to join us to the 2023 On-site PDM3 hoping that it the will be even more successful than the events of recent years.1-5
Acknowledgments
We thank Attendees, Speakers, Chairs and Organizers, who succeed in dealing with new problems even after those of the COVID pandemics became a minor treat.
All Attendees of the 2022 On-site PDM3 thanks the Gastaldello Family, owners, and the staffs of the Hotel Petrarca, Montergrotto, Thermae of Euganean Hills, Padua, Italy for the family atmosphere and the professional managements of all Hotel Services.
List of acronyms
BAM
Basic and Applied Myology
EJTM
European Journal of Translational Myology
MDPI
Molecular Diversity Preservation International
PDM3
Padua Days on Muscle & Mobility Medicine
PMDs
Padua Muscle Days
Funding Statement
Funding: The 2022 PMD3 are supported by the University of Florida Myology Institute and Wellstone Center, Gainesville, FL, USA and by Physiko- & Rheuma-therapie, Institute for Physical Medicine and Rehabilitation, St. Pölten, Austria, Centre of Active Ageing—Competence Centre for Health, Prevention and Active Ageing, St. Pölten, Austria and Ludwig Boltzmann Institute for Rehabilitation Research, St. Pölten, Austria.
Contributor Information
Frank Bittmann, Email: bittmann@uni-potsdam.de.
Elena Ivanova, Email: IvanovaEP@nmicrk.ru.
Halldór Jónsson Jr, Email: halldor@landspitali.is.
1.Sweeney HL, Masiero S, Carraro U.
The 2022 On-site Padua Days on Muscle and Mobility Medicine hosts the University of Florida Institute of Myology and the Wellstone Center, March 30 - April 3, 2022 at the University of Padua and Thermae of Euganean Hills, Padua, Italy: The collection of abstracts. Eur J Transl Myol. 2022. Mar 10;32(1). doi: 10.4081/ejtm.2022.10440 [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Carraro U, Yablonka-Reuveni Z.
Translational research on Myology and Mobility Medicine: 2021 semi-virtual PDM3 from Thermae of Euganean Hills, May 26-29, 2021. Eur J Transl Myol. 2021. Mar 18;31(1):9743. doi: 10.4081/ejtm.2021.9743. [DOI] [PMC free article] [PubMed] [Google Scholar]
3.Carraro U. 30 Years of Translational Mobility Medicine: 2020 Padua Muscle Days go virtual from Euganean Hills, November 19th to 21st. Eur J Transl Myol
2020; 30(4): 9437. doi: 10.4081/ejtm.2020.9437 [DOI] [PMC free article] [PubMed] [Google Scholar]
4.Carraro U. 2020 PMD, 30-years of Translational Mobility Medicine at the time of COVID-19 outbreak: Last-minute forewords from the editor. Eur J Transl Myol. 2020. Apr 1;30(1):8966. doi: 10.4081/ejtm.2019.8966. eCollection 2020 Apr 7. [DOI] [PMC free article] [PubMed] [Google Scholar]
5.Carraro U. Thirty years of translational research in Mobility Medicine: Collection of Abstracts of the 2020 Padua Muscle Days. Eur J Transl Myol
2020;30:3-47. Doi: 10.4081/ejtm.2019.8826. [DOI] [PMC free article] [PubMed] [Google Scholar]
1Department of Sports and Health Sciences, Section Regulative and Preventive Physiology, University of Potsdam, Potsdam, Germany. Emails: Frank Bittmann:
bittmann@uni-potsdam.de
1Department of Sports and Health Sciences, Section Regulative and Preventive Physiology, University of Potsdam, Potsdam, Germany. Emails: Frank Bittmann:
bittmann@uni-potsdam.de
1Department of Sports and Health Sciences, Section Regulative and Preventive Physiology, University of Potsdam, Potsdam, Germany. Emails: Frank Bittmann:
bittmann@uni-potsdam.de
Safely back from the impressive Padua Days we once more like to thank you for giving us the opportunity to take part in the event. We felt the combination of such high internationality on a high scientific level with the family atmosphere is really unique. Congratulations on this successful event and again many thanks for integrating us into the community.We hope you will find some rest for recovery after the intensive days.
1Department of International Cooperation, National Medical Research Center of Rehabilitation and Balneology, Ministry of Healthcare of Russian Federation. New Arbat str. 32, Moscow 121099 +74996733999 (ad.103) Mob.: +79853964266
1Department of International Cooperation, National Medical Research Center of Rehabilitation and Balneology, Ministry of Healthcare of Russian Federation. New Arbat str. 32, Moscow 121099 +74996733999 (ad.103) Mob.: +79853964266
GRAZIE MILLE for your great event and your kind cooperation! Hope to continue participating in your Annual PDM3! As I am in the World Dental Federation, Riccardo and I can invite dentists from all over the world for your Dental Session who work in the functional field (muscles of head and neck, and now full body regarding the medical position of dentists).
I want to thank you by all my heart for your embracing welcome of me and my wife. As you know, me and Paolo are very close friends from the first day of our cooperation in Iceland and I have all the time had such a great pleasure working with him and all the clever Italian students. This congress was in general very interesting especially the results from our studies. We have still a lot more to do and therefore we will meet again „next time“! Our stay at the Hotel Terme delle Nazioni was very nice and the distance was of no problem. I hope you are well and recovering after all the heavy work that needs for such a meeting to be successful as this one was!
1Ludwig Boltzmann Institute for Rehabilitation Research and Institute for Physical Medicine and Rehabilitation, Physiko und Rheumatherapie, St. Pölten, Austria.
1Ludwig Boltzmann Institute for Rehabilitation Research and Institute for Physical Medicine and Rehabilitation, Physiko und Rheumatherapie, St. Pölten, Austria.
Thank you for the excellent organization of the congress bringing together basic science, clinical work and international cooperation. Very impressive was your demonstration that immobilization and inactivity lead to early short-term degeneration of the neuromuscular junction. But you pointed out the importance of the early onset of the translational therapy to avoid/counteract denervation and muscle wasting.
1Department of Aging and Geriatric Research, Division of Biology of Aging, College of Medicine, University of Florida. Clinical and Translational Science Building 3125, 2004 Mowry Rd, PO Box 100107, Gainesville, FL 32611-0107
1Department of Aging and Geriatric Research, Division of Biology of Aging, College of Medicine, University of Florida. Clinical and Translational Science Building 3125, 2004 Mowry Rd, PO Box 100107, Gainesville, FL 32611-0107
What an amazing conference, the science presented was and is at its best! Thanks to Professor Carraro for his leadership in making this meeting happen. Thanks to the local organizing staff and Christa Stout from the Myology Institute to make the program run pretty much flawless. Thanks for tolerating my daughter Arianne Leeuwenburgh playing with Benjamin Mackey in the dining area. They certainly had a blast. Hope to see many of the speakers and attendees next year between Thursday March 28 to Friday March 31, 2023.
After beeing at the Abano Rehabilitation Meeting as one of my first conferences in 1985 and participating in most of the Padua muscle days since, I missed them a lot during the forced break of the past 2 years. Thank you very much for reactivating them On-site with the first window of opportunity and congratulation for the great success. It has been a special highlight, I am sure for all of us, to meet again in this multidisciplinary format for intellectual exchange and optimistic grows of new ideas and joint intentions, in the unique substrate of cultural heritage and hospitality. The Padua-spirit was back present with the first minute, and to be enjoyed throughout the five creative days. Many thanks again and looking forward to the next edition in Spring 2023,
1Research Fellow, Institute of Biological Sciences, University of Leeds, Garstang Building, office 5.59, 6 Claredo Way, Leeds (LS2 9NH), United Kingdon.
1Research Fellow, Institute of Biological Sciences, University of Leeds, Garstang Building, office 5.59, 6 Claredo Way, Leeds (LS2 9NH), United Kingdon.
The On-site Padua Muscle Days 2022 meeting, organized by the University of Padua in collaboration with the University of Florida, was an excellent event from multiple points of view. This meeting, thanks to its vast program, attracted some of the most renowned scientists in the fields of muscle biology, physiology, pathophysiology, therapy and rehabilitation offering incredible research ideas and creating the opportunity to develop prime collaborations. Organizing the conference in a hotel rather than in a convention center allowed the organizers to group all attendees in the same venue for the entire duration of the meeting offering further networking opportunities. Furthermore, opening the conference at the Aula Magna of the University of Padua was very scenic and for the speakers was exciting to have the opportunity to present their work where Galileo, almost 400 years ago, was presenting his results and theories inspiring scholars such as Copernicus. I really thank the organizer for the incredible opportunity.
Just wanted to say thank you! For the wonderful people you have gathered together, for the great scientific discussions, the food, the welcoming venue. It was a great opportunity for me (thanks to Gillian too!) to talk about our latest work and exchange with such an extraordinary international community of muscle experts. Being back to this conference where I started knowing the muscle field, reminded me of the passion that was shared and pushed me to continue investigating the muscle and its fascinating regulatory mehanisms. This event made me appreaciate even more the value of live meetings and I am sure it will be the seed of many great collaborations.
Thank you again for all your infatigable energy and efforts to organize the Padua Muscle Days.
With the warmest regards,
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 3 Abstract 121
Study of correlations between neuro-muscular occlusion and posturegraphic parameters in the elderly for falls prevention: a pilot study
Previous researches have investigated the influence of occlusion on body posture for falls prevention reporting conflicting results. However, a few studies have explored the impact of the occlusion considering the neuromuscular component. Hence, the aim of this study was to investigate any correlations between EMG parameters of masseter and temporal muscles and posturographic parameters in elderly. We enrolled a number of 68 elderly people (16 female and 52 male) without fall history from the geriatric ambulatory clinic of the Department of Internal Medicine and Geriatrics of the University Hospital of Palermo (Italy). All participants were administered a surface EMG assessment of masseter and temporal muscles during a maximum natural intercuspation through a wireless device capable of measure the balancing of dental occlusion (Teethan, Garbagnate Milanese, Milano, Italia) and a baropodometric evaluation to assess plantar pressure in orthostatic position using a baropodometric platform (FreeStep, Sensor Medica, Guidonia Montecelio, Roma, Italia). Our results showed a positive correlation between the percentage of muscle activation of the right temporal muscle and the percentage of pressure on the right forefoot in male participants (r=0.57, p<0.05) and a positive correlation between the percentage of muscle activation of the right temporal muscle and the percentage of pressure on the right rearfoot in female participants (r=0.29, p<0.05). Moreover, for the entire sample, we found a positive correlation between the percentage of muscle activation of the right temporal muscle and the right foot axis (r=0.31, p<0.05). Although further studies are needed, our findings suggest a relationship between plantar pressure and neuromuscular occlusion in elderly without fall history and these may have an implication on falls.
1.Baldini A, Nota A, Tripodi D, Longoni S, Cozza P.
Evaluation of the correlation between dental occlusion and posture using a force platform. Clinics (Sao Paulo). 2013;68(1):45-9. doi: 10.6061/clinics/2013(01)oa07. [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Perinetti G, Marsi L, Castaldo A, Contardo L.
Is postural platform suited to study correlations between the masticatory system and body posture? A study of repeatability and a meta-analysis of reported variations. Prog Orthod. 2012;13(3):273-80. doi: 10.1016/j.pio.2011.12.003. [DOI] [PubMed] [Google Scholar]
3.Bergamini M, Pierleoni F, Gizdulich A, Bergamini C.
Dental occlusion and body posture: a surface EMG study. Cranio. 2008. Jan;26(1):25-32. doi: 10.1179/crn.2008.041. [DOI] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site March 30 Abstract 122
The p97/Nploc4 ATPase complex plays a role in muscle atrophy during cancer and amyotrophic lateral sclerosis
The p97 complex participates in the degradation of muscle proteins during atrophy upon fasting or denervation interacting with different adaptors.1 We investigated whether and how it might also be involved in muscle wasting in cancer, where loss of appetite occurs, or amyotrophic lateral sclerosis (ALS), where motoneuron death causes muscle denervation and fatal paralysis.2 The mRNA levels of p97 were induced in tibialis anterior (TA) of three distinct cancer cachectic mouse models but not in the non-cachectic 4T1 tumor. Similarly, p97 was high both in mRNA and protein in muscles from SOD1G93A mice. Electroporation of a shRNA for murine p97 into muscle reduced the fiber atrophy caused by colon adenocarcinoma C26 and ALS. When we interrogated a microarray we had previously generated for the expression of p97 adaptors, we found few of them induced in cachectic TA from C26-mice. By qPCR, we validated their inductions in TA of cachectic and ALS models and selected Nploc4 as the one most induced. Electroporation of a Crispr/Cas9 vector against Nploc4 into muscle reduced the fiber atrophy caused by C26 and ALS. Since Disulfiram (DSF) uncouples p97 from Nploc4,3 we treated atrophying myotubes with DSF, and found accumulated polyubiquitinated proteins and reduced degradation of long-lived proteins. DSF halves Nploc4 in the soluble muscle fraction and given to C26-mice limited the body and muscle weight loss, with no effect on tumor. The p97/Nploc4 complex plays a crucial role in muscle atrophy during these disorders and disrupting this complex might serve as a novel drug strategy.
The p97 and Nploc4 complex participates in the degradation of muscle proteins during atrophy upon cancer and ALS. Ways to block this complex preserve muscles from wasting in both diseases. The electroporation of a plasmid that silences Nploc4 obviates muscle atrophy in situ in models of cancer cachexia and ALS. The oral administration of Disulfiram (DSF) blocks muscle atrophy in mice with cancer cachexia. The ET compound derived from DSF binds free copper (Cu) and Cu-ET sequesters Nploc4 from p97.
Key Words: Muscle wasting, cancer cachexia, Amyotrophic Lateral Sclerosis, Nploc4, protein degradation
References
1.Piccirillo R, Goldberg AL. The p97/VCP ATPase is critical in muscle atrophy and the accelerated degradation of muscle proteins. EMBO J
2012;31:3334–3350. [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Oskarsson B, Gendron TF, Staff NP. Amyotrophic Lateral Sclerosis: An Update for 2018. Mayo Clin Proc
2018;93:1617–1628. [DOI] [PubMed] [Google Scholar]
3.Skrott Z, Mistrik M, Andersen KK, Friis S, Majera D, Gursky J, et al. Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4. Nature
2017;552:194–199. [DOI] [PMC free article] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 2 Abstract 123
Muscle atrophy and denervation in a mouse model of disuse
Skeletal muscle deconditioning can occur as a consequence of immobilization, disuse or hypoactivity, that are common conditions in patients undergo post-surgery rehabilitation. In response to deconditioning, numerous changes can be detected in the skeletal muscle including atrophy, fiber type shift with metabolic reprograming and neuromuscular junction (NMJ) destabilization.1 In the main studies on animal models and human experiments of muscle deconditioning and unloading, the decrease in strength production is more significant than the reduction in muscle mass. This discrepancy is due to either changes in muscle quality (increases of fat) or in pennation angle, and to E-C uncoupling and denervation.2,3 Several proteins are sound markers of NMJ destabilization and myofiber denervation, such as the C-terminal fragment of Agrin (CAF)4 and the neural cell adhesion molecule (NCAM).5 It has been shown that during disuse or inactivity, circulating levels of CAF are increased and NCAM is relocalized in synaptic and extrasynaptic sites within the myofiber, while rehabilitation protocol of physical exercise recovering skeletal muscle mass and function normalized their expression and distribution.3,4 To increase knowledge in this context, we run a study developing a mouse model of muscle atrophy using a short period of cast immobilization of the lower hindlimb muscles followed by a period of rehabilitation by treadmill running. We will present findings of muscle morphometry, structure and phenotype, either related to denervation and NMJ stability. The results of an AbTrial (Abcam) for the serological assessment of CAF using an ELISA assay untested in murine species will also be discussed.
Key words: Muscle disuse, denervation, C-terminal fragment of Agrin, NCAM
References
1.Arentson-Lantz EJ, English KL, Paddon-Jones D, Fry CS. Fourteen days of bed rest induces a decline in satellite cell content and robust atrophy of skeletal muscle fibers in middle-aged adults. J Appl Physiol (1985). 2016. Apr 15;120(8):965-75. doi: 10.1152/japplphysiol.00799.2015. Epub 2016 Jan 21. [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Monti E, Reggiani C, Franchi MV, Toniolo L, Sandri M, Armani A, Zampieri S, Giacomello E, Sarto F, Sirago G, Murgia M, Nogara L, Marcucci L, Ciciliot S, Šimunic B, Pišot R, Narici MV. Neuromuscular junction instability and altered intracellular calcium handling as early determinants of force loss during unloading in humans. J Physiol. 2021. Jun;599(12):3037-3061. doi: 10.1113/JP281365. Epub 2021 May 19. [DOI] [PMC free article] [PubMed] [Google Scholar]
3.Pietrangelo L, Michelucci A, Ambrogini P, Sartini S, Guarnier FA, Fusella A, Zamparo I, Mammucari C, Protasi F, Boncompagni S.
Muscle activity prevents the uncoupling of mitochondria from Ca2+ Release Units induced by ageing and disuse. Arch Biochem Biophys. 2019. Mar 15;663:22-33. doi: 10.1016/j.abb.2018.12.017. Epub 2018 Dec 20. [DOI] [PMC free article] [PubMed] [Google Scholar]
4.Hettwer S, Dahinden P, Kucsera S, Farina C, Ahmed S, Fariello R, Drey M, Sieber CC, Vrijbloed JW. Elevated levels of a C-terminal agrin fragment identifies a new subset of sarcopenia patients. Exp Gerontol. 2013. Jan;48(1):69-75. doi: 10.1016/j.exger.2012.03.002. Epub 2012 Mar 11. [DOI] [PubMed] [Google Scholar]
5.Covault J, Sanes JR. Neural cell adhesion molecule (N-CAM) accumulates in denervated and paralyzed skeletal muscles. Proc Natl Acad Sci U S A. 1985. Jul;82(13):4544-8. doi: 10.1073/pnas.82.13.4544. [DOI] [PMC free article] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 2 Abstract 124
A new CT analysis of abdominal wall after DIEP flap harvesting
The abdominal microsurgical flap based on the deep inferior epigastric artery perforator (DIEP) flap has become the most popular option worldwide for autologous breast reconstruction. Several authors have investigated the results of reconstructed breasts, but the literature lacks systematic reviews exploring the donor site of the abdominal wall.1-4 To fulfil our aims, a new diagnostic muscle imaging analysis was designed and implemented. This study focused on rectus abdominal muscle morphology and function in a single series of 12 consecutive patients analysed before and after breast reconstruction with a microsurgical DIEP flap. Patients were divided into two groups, namely, “ipsilateral reconstruction” and “contralateral reconstruction”, depending on the side of the flap harvest and breast reconstruction, then evaluated by computed tomography (CT) scans scheduled for tumor staging, and clinically examined by a physiatrist. Numerous alterations in muscle physiology were observed due to surgical dissection of perforator vessels, and rectus muscle distress without functional impairment was a common result. Postoperatively, patients undergoing “contralateral reconstruction” appeared to exhibit fewer rectus muscle alterations. Overall, only three patients were impacted by a long-term deterioration in their quality of life. On the basis of the newly developed and implemented diagnostic approach, we concluded that DIEP microsurgical breast reconstruction is a safe procedure without major complications at the donor site, even if long-term alterations of the rectus muscle are a common finding.
(Upper panel) Pt n#12, preoperative transverse CT scan at sub-umbilical level; (Lower panel) Pt n#12, postoperative transverse CT scan at same level. Yellow dot-ted lines indicate the midline, red dotted arrows indicate the linea alba between the recti muscles.
Keywords: abdominal wall, breast reconstruction, CT, DIEP flap, donor site morbidity, rectus muscle
References
1.Brambullo T, Kohlscheen E, Faccio D, Messana F, Vezzaro R, Pranovi G, Masiero S, Zampieri S, Ravara B, Bassetto F, Vindigni V.
A New CT Analysis of Abdominal Wall after DIEP Flap Harvesting. Diagnostics
2022, 12, 683. doi.org/10.3390/ diagnostics12030683. [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Tan MG, Isaranuwatchai W, DeLyzer T, Butler K, Hofer SOP, O'Neill AC, Zhong T.
A cost-effectiveness analysis of DIEP vs free MS-TRAM flap for microsurgical breast reconstruction. J Surg Oncol. 2019. Mar;119(3):388-396. doi: 10.1002/jso.25325. [DOI] [PubMed] [Google Scholar]
3.Knox ADC, Ho AL, Leung L, Tashakkor AY, Lennox PA, Van Laeken N, Macadam SA. Comparison of Outcomes following Autologous Breast Reconstruction Using the DIEP and Pedicled TRAM Flaps: A 12-Year Clinical Retrospective Study and Literature Review. Plast Reconstr Surg. 2016. Jul;138(1):16-28. doi: 10.1097/PRS.0000000000001747. [DOI] [PubMed] [Google Scholar]
4.Stecco C, Azzena GP, Macchi V, Porzionato A, Behr A, Rambaldo A, Tiengo C, De Caro R.
Rectus abdomi-nis muscle innervation: an anatomical study with surgical implications in diep flap harvesting. Surg Ra-diol Anat. 2018. Aug;40(8):865-872. doi: 10.1007/s00276-017-1944-6. [DOI] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 2 Abstract 125
New paradigms for 3D modelling and surgical planning
1Institute of Biomedical and Neural Engineering, Reykjavik University, Reykjavik, Iceland; 2 Landspitali University Hospital, Reykjavik, Iceland Department of Medicine, Orthopedics, University of Iceland, Reykjavik, Iceland
1Institute of Biomedical and Neural Engineering, Reykjavik University, Reykjavik, Iceland; 2 Landspitali University Hospital, Reykjavik, Iceland Department of Medicine, Orthopedics, University of Iceland, Reykjavik, Iceland
The presentation will be give an insight from the ongoing development of 3D modelling, 3D printing and Digital Anatomy (DA). The DA process describe the mechanism of how human body structures are converted, first into a computer-accessible format and then into a physical model with mimicking not only the geometry but also the structure.1,2 The applications of such technology are not only employed surgical planning, surgeon training, patient communication but also the study of structural and mechanical proprieties on bones, muscles, blood vessels and cartilages. In this way, patient specific conditions such as degeneration, pathologies and tissue mechanical and elastic conditions can be studied like never before.3,4
Reykjavik University will be the 1st place in north Europe operating with this new technology having as aim to develop towards new paradigms for modelling and surgical planning.
Keywords: 3d model, digital anatomy, 3d printing, medical imaging
References
1.Liaw CY, Guvendiren M.
Current and emerging applications of 3D printing in medicine. Biofabrication. 2017. Jun 7;9(2):024102. doi: 10.1088/1758-5090/aa7279. [DOI] [PubMed] [Google Scholar]
2.Mitsouras D, Liacouras P, Imanzadeh A, Giannopoulos AA, Cai T, Kumamaru KK, George E, Wake N, Caterson EJ, Pomahac B, Ho VB, Grant GT, Rybicki FJ. Medical 3D Printing for the Radiologist. Radiographics. 2015. Nov-Dec;35(7):1965-88. doi: 10.1148/rg.2015140320 [DOI] [PMC free article] [PubMed] [Google Scholar]
3.Gargiulo P, Árnadóttir Í, Gíslason M, Edmunds K, Ólafsson I.
New Directions in 3D Medical Modeling: 3D-Printing Anatomy and Functions in Neurosurgical Planning. J Healthc Eng. 2017;2017:1439643. doi: 10.1155/2017/1439643. Epub 2017 Jun 8. [DOI] [PMC free article] [PubMed] [Google Scholar]
4.Recenti M, Ricciardi C, Edmunds K, Jacob D, Gambacorta M, Gargiulo P.
Testing soft tissue radiodensity parameters interplay with age and self-reported physical activity. Eur J Transl Myol. 2021. Jul 12. doi: 10.4081/ejtm.2021.9929. Epub ahead of print [DOI] [PMC free article] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 1 Abstract 126
Mosole’s evidence of transforming muscle fibers coexpressing fast and slow myosin heavy chains in slow type muscle-groupings of life-long active seniors
We like to use the words of the first of us, taken from a review published in this Issue of EJTM 32(1), 2022.1 “With aging, type 2 fast fibers preferentially undergo denervation and are reinnervated by slow-twitch motor neurons. They spread forming new neuro-muscular junctions with the denervated fibers: the result is an increased proportion of slow fibers that group together since they are associated in the same motor unit.
Grouping and fiber type shifting are indeed major histological features of aging skeletal muscle. Exercise has been proposed as an intervention for age-related sarcopenia due to its numerous beneficial effects on muscle mechanical and biochemical features. In 2013, a precursor study in humans was published in the European Journal of Translation Myology (formerly known as Basic and Applied Myology), highlighting the occurrence of reinnervation in the musculature of aged, exercise-trained individuals as compared to matching control. This paper, entitled Reinnervation of Vastus lateralis is increased significantly in seniors (70-years old) with a lifelong history of high-level exercise, is now being reprinted as an «Ejtm Seminal Paper».2,3 In this short review we discuss those results in the light of the most recent advances confirming the occurrence of exercise-mediated reinnervation, ultimately preserving muscle structure and function in elderly people who exercise.”1 The figure is taken from the 2014Mosole’s paper: Mosole S, et al. J Neuropathol Exp Neurol. 2014 Apr;73(4):284-94.4
(A-D) Immunofluorescence staining for fast (A, C) and slow (B, D) myosin heavy chain (MHC) proteins in serial sections of biopsies from sedentary seniors (A, B) and physically active seniors (C, D). White arrows point to small angulated muscle fibers; white circles surround the central fibers that delineate fiber-type groupings. Note that the clustered fibers in the biopsies of the sedentary seniors are of the fast type, whereas those of the physically active seniors are of the slow type. Figure redrawn from Mosole S, et al. J Neuropathol Exp Neurol. 2014 Apr;73(4):284-94.4
Key Words: Aging, coexpression of fast and slow myosin heavy chains, denervation and reinnervation, fiber-type grouping, human skeletal muscle, recreational sport activity
References
1.Coletti C, Acosta GF, Keslacy S, Coletti D.
Exercise-mediated reinnervation of skeletal muscle in elderly people: An update. Eur J Transl Myol. 2022; 32(1): 10416, doi: 10.4081/ejtm.2022.10416. [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Mosole S, Rossini K, Kern H, Löfler S, Simone Fruhmann H, Vogelauer M, Burggraf S, Grim-Stieger M, Cvečka J, Hamar D, Sedliak M, Šarabon N, Pond A, Biral D, Carraro U, Zampieri S.
Reinnervation of Vastus lateralis is increased significantly in seniors (70-years old) with a lifelong history of high-level exercise. Eur J Transl Myol - Basic Appl Myol. 2013;23(4):205–10. [DOI] [PMC free article] [PubMed] [Google Scholar]
3.Mosole S, Carraro U, Kern H, Loefler S, Zampieri S.
Use it or Lose It: Tonic Activity of Slow Motoneurons Promotes Their Survival and Preferentially Increases Slow Fiber-Type Groupings in Muscles of Old Lifelong Recreational Sportsmen. Eur J Transl Myol. 2016. Nov 25;26(4):5972. doi: 10.4081/ejtm.2016.5972. eCollection 2016 Sep 15. [DOI] [PMC free article] [PubMed] [Google Scholar]
4.Mosole S, Carraro U, Kern H, Loefler S, Fruhmann H, Vogelauer M, Burggraf S, Mayr W, Krenn M, Paternostro-Sluga T, Hamar D, Cvecka J, Sedliak M, Tirpakova V, Sarabon N, Musarò A, Sandri M, Protasi F, Nori A, Pond A, Zampieri S. Long-term high-level exercise promotes muscle reinnervation with age. J Neuropathol Exp Neurol. 2014. Apr;73(4):284-94. doi: 10.1097/NEN.0000000000 000032. [DOI] [PubMed] [Google Scholar]
5.Carraro U, Kern H, Gava P, Hofer C, Loefler S, Gargiulo P, Edmunds K, Árnadóttir ÍD, Zampieri S, Ravara B, Gava F, Nori A, Gobbo V, Masiero S, Marcante A, Baba A, Piccione F, Schils S, Pond A, Mosole S. Recovery from muscle weakness by exercise and FES: lessons from Masters, active or sedentary seniors and SCI patients. Aging Clin Exp Res. 2017. Aug;29(4):579-590. doi: 10.1007/s40520-016-0619-1. Epub 2016 Sep 3. [DOI] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 1 Abstract 127
Multi-scale Bone Remodeling Prediction in Patients Undergoing Total Hip Arthroplasty
Total hip arthroplasty (THA) is the most widely used surgery in case of severe damage of hip joints. However, the implant produces significant variations of stress distribution in the operated femur, determining the host bone remodeling, which, in turn, influences the bone quality of THA patient. The aim of the study is to create a prediction model, which allows determining significant parameters for THA femur quality during the remodeling, starting from patient clinical data, diagnostic x-ray computed tomography (XCT) and 3D high resolution imaging. Patient clinical data as age, gender, body mass index, type of operation (unilateral or bilateral), prosthesis (cemented or uncemented) and diagnosed pathologies connected to bone tissue, are correlated to multi-scale parameters of femur. Information at macro level is obtained by the analysis of diagnostic X-ray computed tomography (d-XCT) scans of patient femurs. Data are segmented to collect the densitometry of regions of orthopedic interest (gruen zones). The densitometry of rectus femoris muscle is also considered, due to the fact that remains roughly recognizable also when it is degenerated. Micro and nano femur morphometric parameters are obtained by high resolution 3D imaging on femur biopsies, achieving information on bone microarchitecture and ultrastructure. After a learning, based on above-mentioned THA patients’ data at 24 hours, 1 years and 6 years after the implant, the prediction model can predict the bone quality and the better-regenerated zones. The prediction model of THA femur quality works also as support of further clinical decisions on post-surgery treatments and rehabilitation, by providing a projection on patient mobility.
The figure shows the healthy and operated femur of the same patient. In both are visible the Gruen zones highlighted cutting different planes of the bone, and the rectus femoris muscle. The Bone Mineral Density of those areas in the operated leg, is analyzed for predictions on bone remodeling due to variation of stress distribution after a THA surgery, while healthy leg is used as control.
Key Words: THA, Artificial Intelligence, bone remodeling
References
1.Ciliberti FK, Guerrini L, Gunnarsson AE, Recenti M, Jacob D, Cangiano V, Tesfahunegn YA, Islind AS, Tortorella F, Tsirilaki M, Jónsson H Jr, Gargiulo P, Aubonnet R.
CT- and MRI-Based 3D Reconstruction of Knee Joint to Assess Cartilage and Bone. Diagnostics (Basel). 2022. Jan 22;12(2):279. doi: 10.3390/diagnostics12020279. [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Iuppariello L, Esposito L, Gargiulo P, Gíslason MK, Jónsson H, Sarno A, Cristofolini L, Bifulco P.
A CT-based method to compute femur remodelling after total hip arthroplasty. Computer Methods in Biomechanics and Biomedical Engineering: Imaging &Visualization
2021; 9:4, 428-437. doi: 10.1080/216 81163.2020.1835540. [Google Scholar]
Physical inactivity is a global pandemic that not only causes morbidity and mortality, but also represents a major economic burden worldwide. As a longer-term goal, we must strive to integrate physical activity into our everyday lives.1 Increased physical activity, among other things, has an influence on chronic pain of the locomotor system, minimizes the risk of cardiovascular disease, increases self-esteem and cognitive abilities, maintains mobility and autonomy, and thus has direct effects on healthy life years and direct and indirect health expenditure. The Centre of Active Ageing (CAA) project, funded by the EU cross border cooperation program INTERREG, is intended to be in line with the Austrian health targets (R-GZ) for Austria,2 the National Health Program of Slovakia,3 the recommendations of the Lancet Physical Activity Series Executive Committee, and of the WHO.5 Encouraging policymakers to take physical activity seriously, to motivate people, and to create the opportunities to do so on a regular basis. Preliminary results after 100 subjects finished a 12 week training and education program including 50min strength training and gymnastic sessions twice a week show a consistant improvement in functional performance. Before and after the intervention subjects were tested for upper body strength with handgrip and 30s arm curl test, lower extremity strength/function with isometric knee extension torque and 30s chair stand test and general functional abilities with 2min step test, sit and reach, back scratch, up-and-go test, 10m walking test and questionnaires for general health EQ-5D-5L and physical activity GPAQ. Moreover results of a subgroup which performed a follow-up after six months show a significant improvement which was maintained (Figure). In conclusion, the preliminary results indicate the efficacy of the exercise program within the Centre of Active Aging in St. Pölten for improving physical fitness of elderly.
Funding: European cross border cooperation program INTERREG Slovakia-Austria (project CAA B205; 305041X157)
Key Words: Aging, Physical Activity, Centre of Active Ageing, Elderly
References
1.Andersen LB, Mota J, Di Pietro L.
Update on the global pandemic of physical inactivity. Lancet. 2016;388(10051):1255-6. [DOI] [PubMed] [Google Scholar]
2.Health Targets Austria. Austrian Federal Ministry of Health and Women’s Affairs. 2017 [Google Scholar]
3.National Health Promotion Programme (Slovakia) - Update [Internet]. Ministry of Health of the Slovak Republic; 2014 [Google Scholar]
4.Das P, Horton R.
Physical activity - time to take it seriously and regularly. Lancet. 2016;388(10051):1254-5.e. [DOI] [PubMed] [Google Scholar]
5.Physical activity strategy for the WHO European Region 2016–2025 [Internet]. WHO; 2016.1. [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 1 Abstract 129
Exercise intervention in elderly: a novel system within the Centre of Active Aging in Bratislava
Aging is a complex process generally defined as gradual changes in biological, psychological, sociological domains of life as people grow older. One of the most efficient methods to counteract age-related changes in muscle mass and function is physical exercise. Regular physical activity is known to improve both, motor functions and overall physical fitness. The Center of active aging in Bratislava as a part of the Slovak – Austrian cross-border project “Center of active aging” provides Physical activity and health promotion program; a comprehensive training and education program with the main goal to enhance the overall physical fitness of elderly people and support their health status. The exercise system applied in Centre of active aging is defined as a supervised multimodal training containing functional resistance training mainly in closed kinetic chain and combination of endurance and coordination in water and/or land based. One hundred sixty three participants (n = 163, 74,1 ± 2,97 years) underwent a 10-week (2 times per week) period of multimodal training. Before and after the intervention subjects were tested for lower body strength by 30 s chair-rising test, for maximal walking speed over 10 m and aerobic capacity by the 6-min. bicycle test. After the intervention, in 30 s chair-rising test the number of sit-to-stand repetitions increased from 14,9 ± 3,7 to 19,5 ± 3,8 (p<0,001). The maximal walking speed test increased by 12,5 % (p<0,001) after the intervention. After the training the aerobic capacity in 6-min. bicycle test increased by 5,63 % (p<0,001). In conclusion, the preliminary results indicate the efficacy of the novel exercise system within the Centre of Active Aging in Bratislava for improving physical fitness of elderly.
The project Centre of Active Aging (registration number 305041X157) is funded and supported by European development fund, cross-border cooperation program Interreg Slovakia – Austria.
Changes in maximal gait speed test physical activity program.
Key Words: Aging performance decline, Centre of Active Aging, Elderly
References
1.Haskell WL. J.B. Wolffe Memorial Lecture. Health consequences of physical activity: understanding and challenges regarding dose-response. Med Sci Sports Exerc. 1994. Jun;26(6):649-60. doi: 10.1249/0000 5768-199406000-00001. [DOI] [PubMed] [Google Scholar]
2.Hyatt RH, Whitelaw MN, Bhat A, Scott S, Maxwell JD. Association of muscle strength with functional status of elderly people. Age Ageing. 1990 Sep;19(5):330-6. doi: 10.1093/ageing/19.5.330. [DOI] [PubMed] [Google Scholar]
3.Mitchell WK, Williams J, Atherton P, Larvin M, Lund J, Narici M.
Sarcopenia, dynapenia, and the impact of advancing age on human skeletal muscle size and strength; a quantitative review. Front Physiol. 2012. Jul 11;3:260. doi: 10.3389/fphys.2012.00260. [DOI] [PMC free article] [PubMed] [Google Scholar]
Eur J Transl Myol. 2022 Apr 13;32(2):10521.
2022PDM3 On-site April 1 Abstract 130
Early Onset of Therapy after Knee or Hip Replacement: AMB-REMOB a new concept in outpatient rehabilitation
Total knee or hip replacements are on the top lists of the major operations in the European Union. Especially in Austria, the incidence of hip and knee replacement is high (299 and 230 per 100 000 inhabitants) in comparison to other European countries. In the recent years, there have been advances in surgical technique as well as in rehabilitation and a few studies within the last years show that after orthopedic surgery aquatic physical therapy can improve function and does not increase the risk of wound-related adverse events and is as effective as land-based therapy in terms of pain, edema, strength, and range of motion in the early postoperative period. The aim of this study is to investigate an alternative and favorable treatment scheme; early outpatient remobilization. The rehabilitation measures include underwater therapy (gymnastics and pressure jet massage and electrotherapy and are applied in the usual time and method. The underwater therapy, about 50 min, is meant to improve joint mobility and decongestive measures. Electrotherapy is used to treat pain, performed with standardized forms of electrical currents. The study will include patients undergoing total knee or hip arthroplasty due to chronic cartilage damage. They will be randomly divided into two groups: a control group in which the conventional time schedule of rehabilitation will be applied, and a study group which will start rehabilitation immediately after discharge from the hospital. Another advantage of this early remobilization close to the patient's home is that patients remain in their familiar social environment and therefore are better supported back into the rapid re-integration of their everyday life. The results of this clinical study will help to optimize the rehabilitation process and program for patients after knee and hip arthroplasty surgery, so that they can return to their daily activities and normal life faster afterwards.
Clinical Trial Registration: German Clinical Trial Register DRKS00028152 and Universal Clinical Trial Number UTN U1111-1275-5181
Funding: European cross border cooperation program INTERREG Slovakia-Austria (project AMB-REMOB 305011AXY3)
Potential new early rehabilitation pathway (green) and standard care pathways currently available in Austria.
Key Words: Outpatient rehabilitation, knee replacement, hip replacement, early remobilization, surgery
References
1.Villalta EM, Peiris CL. Early aquatic physical therapy improves function and does not increase risk of wound-related adverse events for adults after orthopedic surgery: a systematic review and meta-analysis. Arch Phys Med Rehabil. 2013. Jan;94(1):138-48. doi: 10.1016/j.apmr.2012.07.020. Epub 2012 Aug 7. [DOI] [PubMed] [Google Scholar]
2.Gibson AJ, Shields N.
Effects of Aquatic Therapy and Land-Based Therapy versus Land-Based Therapy Alone on Range of Motion, Edema, and Function after Hip or Knee Replacement: A Systematic Review and Meta-analysis. Physiother Can. 2015. Spring;67(2):133-41. doi: 10.3138/ptc.2014-01. [DOI] [PMC free article] [PubMed] [Google Scholar]
3.Bürger W, Dietsche S, Morfeld M, Koch U.
[Outpatient and inpatient orthopaedic rehabilitation--results of a study comparing outcome and costs] Rehabilitation (Stuttg). 2002. Apr-Jun;41(2-3):92-102. German. doi: 10.1055/s-2002-28437. [DOI] [PubMed] [Google Scholar]
