Fig. 1. Neutralization of lentivirus pseudotypes encoding different SARS-CoV-2 spike proteins, against different groups of human sera collected after vaccination or primary infection with different variants.

Serum groups are split into sera elicited by infection with different variants (A), and sera elicited by vaccination (B). Variants are ordered according to geometric mean titer (GMT) in D614G sera (panel A, top left), while additional Omicron variants are ordered chronologically. Bold lines with empty circles show the GMTs calculated after estimated differences in individual response size were removed to mitigate biases where not all sera from a group were titrated against a particular variant, as described in Materials and Methods, ‘Titer Analyses’ section. Fainter individual lines and solid points show individual serum titers. Points in the gray region at the bottom of the plots show titers and GMTs that fell below the detection threshold of 20. Each panel is labeled according to the respective serum group and color-coded as indicated on the x-axis. Fig. S2 shows titers split by sample source for the 5 serum groups where samples came from a mixture of cohorts or agencies. Titer box plots, line plots showing the individual serum titers after accounting for individual effects, and titer folddifferences relative to the homologous titer, are shown in figs. S3, S4 and S5 respectively.