Figure 7.

Epistasis analysis of bur2∆ mutants. (a) Telomere lengths of single and double mutant haploid spores were compared by Southern blot analysis. Heterozygous diploids were generated as double mutants with bur2∆ and several genes that affect different pathways of telomere maintenance including tel1∆ (Lanes 7, 8), mre11∆ (Lanes 11, 12), yku80∆ (Lanes 15, 16), rif1∆ (Lanes 19, 20), and tlc1∆ (Lanes 23, 24). In this Southern, as compared to the others in this study using detection of the Y′ region, the telomere G1‐3T repeats are detected using a poly GT probe. (b) Non‐denaturing in‐gel hybridization to examine single‐stranded DNA at telomeres. yku80∆ was used as a control to verify single‐stranded telomeric DNA (Lanes 6, 7, 15). Neither bur2∆, bur1–1, nor bur1–8 showed evidence of increased single‐stranded DNA. Note: A radiolabeled DNA ladder was not included with the probe in the hybridization mix