Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | thinΔA | LaBeau-DiMenna et al., 2012 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-abba | LaBeau-DiMenna et al., 2012 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-mCherry-thin | This study | Stock is available upon request | |
| Genetic reagent (Drosophila melanogaster) | GluRIIASP16 | Petersen et al., 1997 | ||
| Genetic reagent (Drosophila melanogaster) | dysbindin1 | Dickman and Davis, 2009 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-thinRNAi | Perkins et al., 2015 | RRID:BDSC_42826 | |
| Genetic reagent (Drosophila melanogaster) | UAS-mCherryRNAi (P{VALIUM20-mCherry}attP2) | Bloomington Drosophila Stock Center | RRID:BDSC_35785 | |
| Genetic reagent (Drosophila melanogaster) | UAS-venus-dysbindin | Dickman and Davis, 2009 | ||
| Genetic reagent (Drosophila melanogaster) | elavc155-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_458 | |
| Genetic reagent (Drosophila melanogaster) | 24B-Gal4 | Bloomington Drosophila Stock Center | RRID:BDSC_1767 | |
| Antibody | anti-Bruchpilot (nc82) (mouse monoclonal) | DSHB, University of Iowa, USA | RRID:AB_2314866 | (1:100) |
| Antibody | anti-GFP (rabbit polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# G10362, RRID:AB_2536526 | IF: (1:500) WB: (1:1000) |
| Antibody | anti-GFP (mouse mono clonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A-11120, RRID:AB_221568 | (1:500) |
| Antibody | anti-DsRed (mouse monoclonal) | Santa Cruz Biotechnology | Santa Cruz Biotechnology Cat# sc-390909, RRID:AB_2801575 | (1:500) |
| Antibody | anti-SYNORF1 (Synapsin, 3C11) (mouse monoclonal) | DSHB, University of Iowa, USA | RRID:AB_528479 | (1:250) |
| Antibody | anti-Thin (guinea pig polyclonal) | LaBeau-DiMenna et al., 2012 | Larva: (1:200) S2: (1:400) |
|
| Antibody | anti-HRP Alexa-Fluor 647 (goat polyclonal) | Jackson ImmunoResearch Labs | Jackson ImmunoResearch Labs Cat# 123-605-021, RRID:AB_2338967 | (1:200) |
| Antibody | Anti-HA (mouse monoclonal) | Biolegend | BioLegend Cat# 901533, RRID:AB_2801249 | (1:1000) |
| Antibody | Anti-BetaTubulin (mouse monoclonal) | DSHB, University of Iowa, USA | DSHB Cat# E7, RRID:AB_528499 | (1:1000) |
| Antibody | Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# 31430, RRID:AB_228307 | (1:2000) |
| Antibody | Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# 32460, RRID:AB_1185567 | (1:2000) |
| Antibody | Alexa-Fluor anti-mouse 488 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A32723, RRID:AB_2633275 | (1:500) |
| Antibody | Alexa Fluor anti-guinea pig 555 (goat polyclonal) |
Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A-21435 RRID:AB_2535856 | (1:400) |
| Antibody | Atto 594 conjugated anti-mouse (goat polyclonal) | Sigma-Aldrich | Sigma-Aldrich Cat# 76,085 | (1:100) |
| Antibody | Abberior STAR 635P (goat polyclonal) | Abberior | Abberior Cat# ST635P-1002-500 UG, RRID:AB_2893229 | (1:100) |
| Chemical compound, drug | Bouin’s fixative | Sigma-Aldrich | HT-10132 | |
| Chemical compound, drug | Ethanol | Merck | CAS# 64-17-5 | |
| Chemical compound, drug | ProLong Gold Antifade | Thermo Fisher Scientific | P36930 | |
| Chemical compound, drug | Philanthotoxin-433 | Santa Cruz Biotechnology | Cat# sc-255421 | |
| Chemical compound, drug | Schneider’s Drosophila medium | Gibco | Cat# 21720024 | |
| Chemical compound, drug | FuGENE HD | Promega | Cat# E2311 | |
| Chemical compound, drug | Paraformaldehyde | Merck | HT501128 | |
| Chemical compound, drug | NP-40 | Thermo Fisher Scientific | 85,125 | |
| Chemical compound, drug | Deoxycholate | Sigma-Aldrich | D6750 | |
| Chemical compound, drug | cOmplete | Sigma-Aldrich | 11697498001 | |
| Chemical compound, drug | ECL Prime Western Blotting Detection Reagent | GE Healthcare | Cat# 28980926 | |
| Cell line (D. melanogaster) | Drosophila Schneider 2 (S2) Cells | Thermo Fisher Scientific | Cat# R69007 | |
| Commercial assay, kit | Nitrocellulose membrane | Amersham Hibond GE Healthcare | Cat# 88,018 | |
| Recombinant DNA reagent | pMT-Gal4 | Addgene | RRID:Addgene_53366 | |
| Software, algorithm | Fiji / ImageJ | https://fiji.sc | RRID:SCR_002285 | Version 1.51n |
| Software, algorithm | Clampex | Axon CNS, Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | Leica Application Suite X | Leica Microsystems | RRID:SCR_013673 | |
| Software, algorithm | Huygens Software | https://svi.nl/HuygensSoftware | RRID:SCR_014237 | |
| Software, algorithm | Igor Pro | WaveMetrics | RRID:SCR_000325 | Version 6.37 |
| Software, algorithm | NeuroMatic | Rothman and Silver, 2018 | RRID:SCR_004186 | Version 3.0c |
| Software, algorithm | NumPy | https://www.numpy.org | RRID:SCR_008633 | |
| Software, algorithm | SciPy | https://www.scipy.org | RRID:SCR_008058 | |
| Software, algorithm | IPython | http://ipython.org | RRID:SCR_001658 | |
| Software, algorithm | Neo | http://neuralensemble.org/neo | RRID:SCR_000634 | |
| Software, algorithm | Shapely | (Gillies, 2007) https://github.com/shapely/shapely | ||
| Software, algorithm | RStudio | (R Studio Team, 2020) http://www.rstudio.com/ |
RRID:SCR_000432 | Version 2021.09.0 |
| Software, algorithm | pwr-package | (Champely, 2020) https://github.com/heliosdrm/pwr |
||
| Software, algorithm | GNU Image Manipulation Program | https://www.gimp.org/ | RRID:SCR_003182 | Version 2.8.10 |
| Software, algorithm | Inkscape | http://www.inkscape.org | RRID:SCR_014479 | Version 0.92.2 |
| Software, algorithm | Affinity Designer | https://affinity.serif.com/en-us/designer/ | RRID:SCR_016952 | Version 1.10.4 |