Figure 4.

Regions of a 2D NOESY spectrum (300 ms mixing time, 30 °C) of QD3‐sbl (1 mM) in the presence of 1 equiv. SYUIQ‐5. (A) H6/8(ω₂)−H3’(ω₁) and (B) H6/8(ω₂)−H1’(ω₁) spectral region; continuous networks of base‐sugar resonances are followed by vertical and horizontal lines with NOE connectivities in the duplex domain traced by blue lines; intra‐nucleotide cross‐peaks are labeled by residue number. (C) H8/6/2(ω₂)−H1(ω₁) spectral region; intra‐tetrad H8(ω₂)−H1(ω₁) cross‐peaks are labeled with colors depending on G‐tetrad layer; inter‐tetrad connectivities along the G‐columns are traced by horizontal and vertical lines and intermolecular contacts between quadruplex imino and ligand protons are labeled in blue.