FIGURE 5.

The expression level of tyrosine hydroxylase (TH) in FluoroGold‐labeled cells in the ventral tegmental area (VTA). Dopaminergic projections to the nucleus accumbens (NAcc) were labeled with both the retrograde tracer FluoroGold injected into the NAcc and TH. Representative immunofluorescence staining of FluoroGold‐positive neurons ((A) control group for central infused of FluoroGold for immunofluorescence analysis (Con‐IFG), (B) nonorganic erectile dysfunction for immunofluorescence analysis (nED‐IFG)), TH‐positive neurons ((C) Con‐IFG, (D) nED‐IFG), and FluoroGold + TH‐positive neurons ((E) Con‐IFG, (F) nED‐IFG) (indicated by arrows). Scale bar indicates 20 μm