Figure 2.

Example of NSCLC tumoroid model and its applications. Tumor samples were obtained from NHC (Nouvel Hôpital Civil) in Strasbourg, France. Washing and enzymatic digestion were performed within 1–2 h after resection. Tumoral cells were centrifuged, and other cell types, such as cancer-associated fibroblasts (CAFs) were added to reconstitute the TME complexity. Tumoroids were grown in a final volume of 200 μL in ultra-low attachment 96-well plates, forming after 5 days of incubation at 37 °C. This tumoroid model is aimed at being inserted into a microfluidic chip to overcome the limit of the static 3D organoid model. Drug toxicity and efficacy assessment are facilitated to select the best treatment opportunity for each individual patient. Created with BioRender.com.