FIGURE 4.

Microglia affect the expression of oxidative stress and immune response‐related genes in assembloids. (a) Differentially expressed gene enrichment analysis in assembloids against midbrain organoids reveals 12 significant network process pathways (FDR <0.05). (b) Expression of genes related to response to oxidative stress in assembloids compared with midbrain organoids. (c) Gene expression of genes related to immune response of assembloids. The presence of microglia increases the expression of genes related to antigen presentation and immune response, and decreases the expression of those related to autophagy in non‐microglia cells. Box plots show mean expression and SD. (d) Enrichment analysis of cluster specific DEG p <.05 between assembloids and midbrain organoids reveals significant FDR <0.05 network processes involved in oxidative stress, immune response as well as synaptic regulation. Dots represent single cells. Data are represented as mean ± SD. *p <.05, **p <.01, ***p <.001, ****p <.0001 using a Wilcox test