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. 2022 Jul 14;27(14):4494. doi: 10.3390/molecules27144494

Figure 3.

Figure 3

Biophysical and biochemical analysis of bacterial TIR-containing proteins. (A) The 15N-HSQC spectra of the tirA-TIR E206A mutant. (B) The 15N-HSQC spectra of GB1 alone (gray) and GB1-tirE-TIR (red) with an enlargement including the additional spectrum of GB1-tirE-TIR incubated with saturating concentrations of NAD+ (blue). (C) Analytical sizing of full-length tirS (red), tirS-CC (blue), and the tirS-TIR E216A mutant (green). (D) Analytical sizing of full-length tirA (red), tirA-CC (blue), and the tirA-TIR E206A mutant (green). (E) Analytical sizing of full-length tirE (red), tirE-CC (blue) and GB1-tirE-TIR (green). Sample loads were all 100 mL of 200 μM, except tirS-TIR E216A and tirS-TIR E206A, which were 20 μM and 50 μM, respectively.